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首页> 外文期刊>Cancer prevention research. >Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia
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Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

机译：哥伦比亚年轻女性HPV DNA检测和分型的尿液和宫颈样品的比较

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Urine sampling for HPV DNA detection has been proposed as an effective method for monitoring the impact of HPV vaccination programs; however, conflicting results have been reported. The goal of this study was to evaluate the performance of optimized urine HPV DNA testing in women aged 19 to 25 years. Optimization process included the use of first void urine, immediate mixing of urine with DNA preservative, and the concentration of all HPV DNA, including cell-free DNA fragments. Urine and cervical samples were collected from 535 young women attending cervical screening at health centers from two Colombian cities. HPV DNA detection and genotyping was performed using an HPV type-specific multiplex genotyping assay, which combines multiplex polymerase chain reaction with bead-based Luminex technology. Concordance between HPV DNA detection in urine and cervical samples was determined using kappa statistics and McNemar tests. The accuracy of HPV DNA testing in urine samples was evaluated measuring sensitivity and specificity using as reference the results obtained from cervical samples. Statistical analysis was performed using STATA11.2 software. The findings revealed an overall HPV prevalence of 60.00% in cervical samples and 64.72% in urine samples, HPV-16 being the most frequent HPV type detected in both specimens. Moreover, our results indicate that detection of HPV DNA in first void urine provides similar results to those obtained with cervical samples and can be used to monitor HPV vaccination trials and programs as evidenced by the substantial concordance found for the detection of the four vaccine types. (C) 2016 AACR.
机译：已经提出了用于HPV DNA检测的尿液采样作为监测HPV疫苗接种计划影响的有效方法。然而，据报道结果相互矛盾。这项研究的目的是评估19至25岁女性的最佳尿液HPV DNA检测性能。优化过程包括使用第一次排尿，立即将尿液与DNA防腐剂混合，以及所有HPV DNA的浓度，包括无细胞DNA片段。从哥伦比亚两个城市的卫生中心接受宫颈筛查的535名年轻妇女中收集了尿液和宫颈样本。 HPV DNA检测和基因分型使用HPV类型特异性多重基因分型检测方法进行，该方法将多重聚合酶链反应与基于微珠的Luminex技术相结合。尿液和宫颈样品中HPV DNA检测的一致性使用kappa统计量和McNemar测试确定。评估尿液中HPV DNA检测的准确性，并以从宫颈样品中获得的结果为参考，评估其敏感性和特异性。使用STATA11.2软件进行统计分析。调查结果显示，宫颈样本中的HPV总体患病率为60.00％，尿液样本中的HPV患病率为64.72％，HPV-16是两个样本中检测到的最常见的HPV类型。此外，我们的结果表明，在第一个空尿中检测HPV DNA提供的结果与宫颈样本获得的结果相似，并且可以用于监测HPV疫苗接种试验和程序，这在检测四种疫苗类型方面取得了实质性的一致性证明了这一点。 （C）2016 AACR。

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