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首页> 外文期刊>Molecular medicine reports >Effects of Asiasari radix on the morphology and viability of mesenchymal stem cells derived from the gingiva
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Effects of Asiasari radix on the morphology and viability of mesenchymal stem cells derived from the gingiva

机译：白s对牙龈间充质干细胞形态和活力的影响

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摘要

Medicinal herbs used in traditional Oriental medicine, which have been in use clinically for thousands of years, are attractive sources of novel therapeutics or preventatives. Asiasari radix (A. radix) has been suggested for use in the treatment of dental diseases, including toothache and aphthous stomatitis. The aim of this study was to evaluate the effects of A. radix extracts on the morphology and viability of human stem cells derived from the gingiva. An Asiasarum heterotropoides extract was centrifuged and freeze-dried in a lyophilizer. Stem cells derived from the gingiva were grown in the presence of A. radix at concentrations ranging between 0.1 mu g/ml and 1 mg/ml (0, 0.1, 1, 10, 100 and 1,000 mu g/ml). Cell morphology was evaluated with an optical microscope and the viability of the cells was quantitatively analyzed with a cell counting kit-8 (CCK-8) assay for up to seven days. The untreated control group exhibited normal fibroblast morphology. The shapes of the cells following 0.1, 1, 10 and 100 mu g/ml A. radix treatments were similar to those of the control group. However, a significant change was noted in the 1,000 mu g/ml group on day 1, when compared with the untreated group. Furthermore, on day 7, the shapes of the cells following 100 and 1,000 mu g/ml A. radix treatments were rounder and fewer cells were present, when compared with those of the control group. The cultures that grew in the presence of A. radix did not exhibit any changes in the CCK-8 assay on day 2; however, significant reductions in cell viability were noticed following 100 and 1,000 mu g/ml A. radix treatment on days 5 and 7. Within the limits of this study, A. radix influenced the viability of the stem cells derived from the gingiva. Thus, the direct application of A. radix to oral tissues may produce adverse effects at high doses. Therefore, the concentration and application time of A. radix requires meticulous control to obtain optimal results. These effects require consideration, if the use of A. radix is planned for the treatment of dental diseases.
机译：在临床上已经使用了数千年的传统东方医学中使用的草药，是新颖疗法或预防剂的诱人来源。已建议将Asiasari radix（A. radix）用于治疗牙齿疾病，包括牙痛和口疮性口炎。这项研究的目的是评估根提取物对源自牙龈的人类干细胞的形态和活力的影响。将亚洲细颈藻的提取物离心并在冻干机中冷冻干燥。来源于牙龈的干细胞在小曲霉存在下以0.1μg/ ml至1mg / ml（0、0.1、1、10、100和1,000μg/ ml）的浓度生长。用光学显微镜评估细胞形态，并使用细胞计数试剂盒8（CCK-8）分析定量分析细胞的活力长达7天。未经处理的对照组表现出正常的成纤维细胞形态。 0.1、1、10和100μg/ ml根处理后的细胞形状与对照组相似。但是，与未治疗组相比，第1天的1000μg/ ml组出现了显着变化。此外，在第7天，与对照组相比，在100和1000μg/ ml基基处理之后，细胞的形状更圆，并且存在的细胞更少。在第2天，在A. radix存在下生长的培养物在CCK-8分析中未表现出任何变化。然而，在第5天和第7天，分别以100和1,000μg/ ml根茎土壤处理后，发现细胞活力显着降低。在本研究的范围内，根基土壤影响了源自牙龈的干细胞的生存能力。因此，将大青根直接施用到口腔组织上可能会产生高剂量的不良反应。因此，农杆菌的浓度和施用时间需要仔细控制以获得最佳结果。如果计划使用基生根治疗牙齿疾病，则需要考虑这些效果。

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