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首页> 外文期刊>Molecular medicine reports >Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells
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Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

机译：Angelicae Dahuricae adix对间充质干细胞形态和活力的评价

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摘要

Angelicae dahuricae radix is a traditional herbal medicine used to treat various diseases in China and Korea, such as colds, headaches, rhinitis and psoriasis. Angelicae dahuricae radix has been used as an anti-inflammatory, analgesic, antipyretic and antioxidant remedy. This study was performed in order to evaluate the effects of the extracts of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells derived from the gingiva. Mesenchymal stem cells derived from the gingiva were grown in the presence of Angelicae dahuricae radix at final concentrations that ranged from 0.001 to 100 g/ml. The morphology of the cells was viewed under an inverted microscope, and the analysis of cell proliferation was performed with cell counting kit-8 (CCK-8) on days 1, 3 and 7 The cells in the control group had spindle-shaped, fibroblast-like morphology at days 1, 3 and 7 under optical microscopy. The shapes of the cells in 0.001, 0.01, 0.1, 1, 10 and 100 g/ml Angelicae dahuricae radix were similar to the shapes of the cells in the control group. The relative values of the CCK-8 assays of 0.001, 0.01, 0.1, 1, 10, and 100 g/ml Angelicae dahuricae radix were 102.5 +/- 0.6, 133.3 +/- 9.6, 148.4 +/- 20.5, 1477 +/- 12.6, 132.3 +/- 277 and 101.1 +/- 46%, respectively, when the CCK-8 result of the control group on day 1 was considered to be 100%. There was a marginal increase in cell proliferation at 0.1 and 1 g/ml groups at day 1; however, this did not achieve statistical signifcance (P=0.052). The relative values of the CCK-8 assays of 0.001, 0.01, 0.1, 1, 10 and 100 g/ml Angelicae dahuricae radix were 96.5 +/- 1.3, 89.3 +/- 0.9, 90.3 +/- 3.0, 84.8 +/- 12.2, 92.3 +/- 4.5 and 86.8 +/- 11.7%, respectively, when the CCK-8 result of the control group on day 3 was considered to be 100% (P>0.05). The relative values of the CCK-8 assays of 0.001, 0.01, 0.1, 1, 10 and 100 g/ml Angelicae dahuricae radix day 7 were 94.9 +/- 22.3, 102.8 +/- 22.1, 127.4 +/- 7.4, 130.4 +/- 1.3, 129.2 +/- 10.8 and 124.8 +/- 9.1%, respectively, when the CCK-8 result of the control group on day 7 was considered to be 100%, but there were no statistically significant differences among the groups (P>0.05). Within the limits of this study, Angelicae dahuricae radix at the tested concentrations did not produce statistically significant differences in the viability of stem cells derived from the gingiva.
机译：Angelicae Dahuricae Radix是一种传统的草药，用于治疗中国和韩国各种疾病，如感冒，头痛，鼻炎和牛皮癣。 Angelicae Dahuricae Radix已被用作抗炎，镇痛，解热和抗氧化剂补救措施。进行该研究，以评估Angelicae Dahuricae adrix提取物对衍生自牙龈的间充质干细胞的形态和活力的影响。衍生自Gingiva的间充质干细胞在Angelicae Dahuricae adrix的存在下以终浓度为0.001至100g / ml而生长。在倒置显微镜下观察细胞的形态，并在第1,3和7天的细胞计数试剂盒-8（CCK-8）进行细胞增殖的分析，对照组中的细胞具有主轴形，成纤维细胞在光学显微镜下第1,3和7天的形态。在0.001,0.01,0.1,1,10和100g / ml Angelicae Dahuricae Dahuricae adix中的细胞形状类似于对照组中的细胞的形状。 0.001,0.01,01,1,10和100g / ml Angelicae Dahuricae adix的CCK-8测定的相对值为102.5 +/- 0.6,133.3 +/- 9.6,148.4 +/- 20.5,1477 + / - 12.6,132.3 +/- 277和101.1 +/- 46％，当第1天的CCK-8结果被认为是100％。第1天在0.1和1g / ml基团下进行边际增加;但是，这没有达到统计学意义（P = 0.052）。 CCK-8测定的相对值为0.001,0.01,0.1,1,10和100g / ml Angicae Dahuricae adix的Dahuricae adix为96.5 +/- 1.3,89.3 +/- 0.9,90.3 +/- 3.0,84.8 +/- 12.2,92.3 +/- 4.5和86.8 +/- 11.7％，当第3天对照组的CCK-8结果被认为是100％（P> 0.05）。 0.001,0.01,0.1,11,10和100g / ml Angelicae Dahuricae Carrix第7天的CCK-8测定的相对值为94.9 +/-22.3,102.8 +/-22.1,127.4 +/- 7.4,130.4 + / - 1.3,129.2 +/- 10.8和124.8 +/- 9.1％，分别当第7天的控制组的CCK-8结果被认为是100％，但群体之间没有统计学意义的差异（ p> 0.05）。在本研究的范围内，在测试浓度下的Angelicae Dahuricae Natix在衍生自牙龈的干细胞的生存率上没有产生统计学上显着的差异。

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