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首页> 外文期刊>Experimental and therapeutic medicine >Evaluation of the effects of Cimicifugae Rhizoma on the morphology and viability of mesenchymal stem cells
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Evaluation of the effects of Cimicifugae Rhizoma on the morphology and viability of mesenchymal stem cells

机译：评价升麻药对间充质干细胞的形态和活力的影响

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Cimicifugae Rhizoma is a traditional herbal medicine used to treat various diseases in Korea, China and Japan. Cimicifugae Rhizoma is primarily derived from Cimicifuga heracleifolia Komarov or Cimicifuga foetida Linnaeus. Cimicifugae Rhizoma has been used as an anti-inflammatory, analgesic and antipyretic remedy. The present study was performed to evaluate the extracts of Cimicifugae Rhizoma on the morphology and viability of human stem cells derived from gingiva. Stem cells derived from gingiva were grown in the presence of Cimicifugae Rhizoma at final concentrations that ranged from 0.001 to 1,000 mu g/ml. The morphology of the cells was viewed under an inverted microscope and the analysis of cell proliferation was performed using a Cell Counting kit-8 (CCK-8) assay on days 1, 3, 5 and 7. Under an optical microscope, the control cells exhibited a spindle-shaped, fibroblast-like morphology. The shapes of the cells in the groups treated with 0.001, 0.01, 0.1, 1 and 10 mu g/ml Cimicifugae Rhizoma were similar to the shapes in the control group. Significant alterations in morphology were noted in the 100 and 1,000 mu g/ml groups when compared with the control group. The cells in the 100 and 1,000 mu g/ml groups were rounder, and fewer cells were present. The cultures that were grown in the presence of Cimicifugae Rhizoma at a concentration of 0.001 mu g/ml on day 1 had an increased CCK-8 value. The cultures grown in the presence of Cimicifugae Rhizoma at a concentration of 10 mu g/ml on day 7 had a reduced CCK-8 value. Within the limits of this study, Cimicifugae Rhizoma influenced the viability of stem cells derived from the gingiva, and its direct application onto oral tissues may have adverse effects at high concentrations. The concentration and application time of Cimicifugae Rhizoma should be meticulously controlled to obtain optimal results.
机译：升麻药是在韩国，中国和日本用于治疗各种疾病的传统草药。升麻（Cimicifugae Rhizoma）主要来源于升麻（Cimicifuga heracleifolia Komarov）或升麻（Cimicifuga foetida Linnaeus）。升麻药已被用作抗炎，止痛和退热药。进行本研究以评价升麻的提取物对源自牙龈的人干细胞的形态和活力的影响。源于牙龈的干细胞在Cimicifugae Rhizoma的存在下生长，最终浓度范围为0.001至1,000μg / ml。在倒置显微镜下观察细胞的形态，并在第1、3、5和7天使用Cell Counting kit-8（CCK-8）分析法进行细胞增殖分析。在光学显微镜下，对照细胞表现出纺锤状的成纤维样形态。 0.001、0.01、0.1、1和10μg/ ml升麻药处理组的细胞形状与对照组相似。与对照组相比，在100和1,000μg / ml组中注意到形态上的显着改变。 100和1,000μg / ml组中的细胞更圆，并且存在的细胞更少。在第1天在浓度为0.001μg/ ml的升麻的情况下生长的培养物具有增加的CCK-8值。在第7天在浓度为10μg/ ml的升麻的存在下生长的培养物具有降低的CCK-8值。在本研究的范围内，升麻药影响了源自牙龈的干细胞的活力，而将其直接施用到口腔组织中可能会产生高浓度的不利影响。应严格控制升麻的浓度和施用时间，以获得最佳效果。

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