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首页> 外文期刊>Molecular medicine reports >Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells
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Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

机译：评价当归对间充质干细胞形态和活力的影响

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摘要

Angelicae dahuricae radix is a traditional herbal medicine used to treat various diseases in China and Korea, such as colds, headaches, rhinitis and psoriasis. Angelicae dahuricae radix has been used as an anti-inflammatory, analgesic, antipyretic and antioxidant remedy. This study was performed in order to evaluate the effects of the extracts of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells derived from the gingiva. Mesenchymal stem cells derived from the gingiva were grown in the presence of Angelicae dahuricae radix at final concentrations that ranged from 0.001 to 100 g/ml. The morphology of the cells was viewed under an inverted microscope, and the analysis of cell proliferation was performed with cell counting kit-8 (CCK-8) on days 1, 3 and 7 The cells in the control group had spindle-shaped, fibroblast-like morphology at days 1, 3 and 7 under optical microscopy. The shapes of the cells in 0.001, 0.01, 0.1, 1, 10 and 100 g/ml Angelicae dahuricae radix were similar to the shapes of the cells in the control group. The relative values of the CCK-8 assays of 0.001, 0.01, 0.1, 1, 10, and 100 g/ml Angelicae dahuricae radix were 102.5 +/- 0.6, 133.3 +/- 9.6, 148.4 +/- 20.5, 1477 +/- 12.6, 132.3 +/- 277 and 101.1 +/- 46%, respectively, when the CCK-8 result of the control group on day 1 was considered to be 100%. There was a marginal increase in cell proliferation at 0.1 and 1 g/ml groups at day 1; however, this did not achieve statistical signifcance (P=0.052). The relative values of the CCK-8 assays of 0.001, 0.01, 0.1, 1, 10 and 100 g/ml Angelicae dahuricae radix were 96.5 +/- 1.3, 89.3 +/- 0.9, 90.3 +/- 3.0, 84.8 +/- 12.2, 92.3 +/- 4.5 and 86.8 +/- 11.7%, respectively, when the CCK-8 result of the control group on day 3 was considered to be 100% (P>0.05). The relative values of the CCK-8 assays of 0.001, 0.01, 0.1, 1, 10 and 100 g/ml Angelicae dahuricae radix day 7 were 94.9 +/- 22.3, 102.8 +/- 22.1, 127.4 +/- 7.4, 130.4 +/- 1.3, 129.2 +/- 10.8 and 124.8 +/- 9.1%, respectively, when the CCK-8 result of the control group on day 7 was considered to be 100%, but there were no statistically significant differences among the groups (P>0.05). Within the limits of this study, Angelicae dahuricae radix at the tested concentrations did not produce statistically significant differences in the viability of stem cells derived from the gingiva.
机译：当归是一种传统草药，用于治疗中国和韩国的各种疾病，例如感冒，头痛，鼻炎和牛皮癣。白da当归已被用作抗炎，止痛，解热和抗氧化剂的药物。进行这项研究是为了评估当归提取物对源自牙龈的间充质干细胞的形态和活力的影响。当归的当归存在下，源自牙龈的间充质干细胞以0.001至100 g / ml的最终浓度生长。在倒置显微镜下观察细胞的形态，并在第1、3和7天用细胞计数试剂盒8（CCK-8）进行细胞增殖分析。对照组中的细胞具有纺锤形的成纤维细胞在第1、3和7天在光学显微镜下观察到类似的形态。 0.001、0.01、0.1、1、10和100 g / ml当归基的细胞形状与对照组中的细胞形状相似。 0.001、0.01、0.1、1、10和100 g / ml当归基数的CCK-8分析的相对值为102.5 +/- 0.6、133.3 +/- 9.6、148.4 +/- 20.5、1477 + / -当将第1天对照组的CCK-8结果视为100％时，分别为12.6、132.3 +/- 277和101.1 +/- 46％。在第1天，0.1和1 g / ml组的细胞增殖略有增加;然而，这没有达到统计学显着性（P = 0.052）。 0.001、0.01、0.1、1、10和100 g / ml当归基数的CCK-8分析的相对值为96.5 +/- 1.3、89.3 +/- 0.9、90.3 +/- 3.0、84.8 +/-当将第3天对照组的CCK-8结果视为100％时，分别为12.2、92.3 +/- 4.5和86.8 +/- 11.7％。第7天0.001、0.01、0.1、1、10和100 g / ml当归的CCK-8分析的相对值为94.9 +/- 22.3、102.8 +/- 22.1、127.4 +/- 7.4、130.4 +当将第7天对照组的CCK-8结果视为100％时，分别为1.3、129.2 +/- 10.8和124.8 +/- 9.1％，但各组之间无统计学差异（ P> 0.05）。在这项研究的范围内，当归浓度的当归基数在牙龈来源的干细胞活力上没有产生统计学上的显着差异。

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