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首页> 外文期刊>Molecular medicine reports >Integrated microRNA-gene analysis of coronary artery disease based on miRNA and gene expression profiles
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Integrated microRNA-gene analysis of coronary artery disease based on miRNA and gene expression profiles

机译：基于miRNA和基因表达谱的冠状动脉疾病microRNA基因整合分析

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摘要

The present study aimed to investigate the key genes and microRNAs (miRNA/miRs) associated with coronary artery disease (CAD) progression. The gene expression profile of GSE20680 and GSE12288, and the miRNA expression profile of GSE28858 were downloaded from the gene expression omnibus database. The differentially expressed genes (DEGs) in GSE20680 and GSE12288, and the differentially expressed miRNAs in GSE28858 were screened using the limma package in R software. Common DEGs between GSE20680 and GSE12288 were selected. Functions and pathways of DEGs and miRNAs were enriched using the DAVID tool from the GO and KEGG databases. The regulatory network of miRNA and selected CAD-associated DEGs was constructed. A total of 270 DEGs (167 upregulated and 103 downregulated) based on the GSE20680 dataset, and 2,268 DEGs (534 upregulated and 1,734 downregulated) based on the GSE12288 dataset, were screened. For the differentially expressed miRNAs, 214 were identified (102 upregulated and 112 downregulated) in CAD samples and were screened. Interferon regulatory factor 2 (IRF2) and cell death-inducing DFFA-like effector b (CIDEB), which are regulated by signal transducer and activator of transcription 3 and myc-associated factor X, were identified as common DEGs for CAD. miR-455-5p, miR-455-3p and miR-1257, which are involved in the major histocompatibility complex (MHC) protein assembly pathway and peptide antigen assembly with MHCclassI protein complex pathway, may regulate various miRNAs and target genes, including pro-opiomelancortin (POMC), toll-like receptor 4 (TLR4), interleukin 10 (IL10), activating transcription factor 6 (ATF6) and calreticulin (CALR). The current study identified IRF2 and CIDEB as crucial genes, and miRNA-455-5p, miRNA-455-3p and miR-1257 along with their target genes POMC, TLR4 and CALR, as miRNAs involved in CAD progression. Thus, the present study may provide a basis for future research into the progression mechanism of CAD.
机译：本研究旨在调查与冠心病（CAD）进展相关的关键基因和microRNA（miRNA / miRs）。 GSE20680和GSE12288的基因表达谱以及GSE28858的miRNA表达谱从基因表达综合数据库下载。使用R软件中的limma软件包筛选了GSE20680和GSE12288中的差异表达基因（DEG），以及GSE28858中的差异表达miRNA。选择了GSE20680和GSE12288之间的通用DEG。使用来自GO和KEGG数据库的DAVID工具丰富了DEG和miRNA的功能和途径。构建了miRNA和选定的CAD相关DEG的调控网络。根据GSE20680数据集总共筛选了270个DEG（上调167个，下调103个），以及基于GSE12288数据集筛选了2268个DEG（上调534个，下调1734个）。对于差异表达的miRNA，在CAD样品中鉴定了214种（上调102种，下调112种），并进行了筛选。干扰素调节因子2（IRF2）和诱导细胞死亡的DFFA样效应物b（CIDEB），由信号转导和转录激活因子3以及myc相关因子X调节，被确定为CAD的常见DEG。 miR-455-5p，miR-455-3p和miR-1257与主要的组织相容性复合物（MHC）蛋白组装途径和具有MHCclassI蛋白复合物途径的肽抗原组装有关，它们可能会调节各种miRNA和靶基因，包括脯氨酸-opiomelancortin（POMC），toll​​样受体4（TLR4），白介素10（IL10），激活转录因子6（ATF6）和钙网蛋白（CALR）。目前的研究确定了IRF2和CIDEB是关键基因，miRNA-455-5p，miRNA-455-3p和miR-1257以及它们的靶基因POMC，TLR4和CALR是参与CAD进展的miRNA。因此，本研究可为以后的CAD进展机制研究提供基础。

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