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首页> 外文期刊>Mutation Research, A. Reviews in Genetic Toxicology >Induction of DNA strand breaks by 3-chloro4-(dichloromethyl)-5-hydroxy-2(5H)-furanone and humic substances in relation to ghitathione and calcium status in human white blood cells
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Induction of DNA strand breaks by 3-chloro4-(dichloromethyl)-5-hydroxy-2(5H)-furanone and humic substances in relation to ghitathione and calcium status in human white blood cells

机译：3-氯4-（二氯甲基）-5-羟基-2（5H）-呋喃酮和腐殖质诱导DNA链断裂与人白细胞中紫杉硫酮和钙状态的关系

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摘要

3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) induced DNA strand-breakage (measured by fluorometric analysis of DNA unwinding) in human white blood cells at sub-cytotoxic doses (1.0-1000 mu M; 60 mm exposure). Although a dose-dependent decrease in glutathione levels was observed with MX, this is not necessarily the causative factor in the observed DNA damage, since no strand breakage Was seen on depletion of cellular, glutathione to 23% of control by diethylmaleate. In addition, the strand scission does not appear to be mediated by elevation of intracellular calcium as no MX-induced release of calcium stores was observed. Strand breakage may, however, be Ca~(2+)-dependent as evidenced by inhibition following deprivation of Ca~(2+) by Quin-2, Chlorinated futvic acids (> 3 mu g/ml) also depleted glutathione and induced strand breaks at sub-cytotoxic doses (up to 300 mu g/ml) on prolonged exposure (60 min), The unchlorinated material was, however, equally able to cause DNA strand breakage(without glutathione depletion), MX appears, therefore, to be only one of a number of components of chlorinated humic substances able to induce DNA strand breakage.
机译：以亚细胞毒性剂量（1.0-）诱导人白血球中3-Chloro-4-（dichloromethyl）-5-hydroxy-2（5H）-呋喃酮（MX）诱导的DNA链断裂（通过DNA展开的荧光分析测量）。 1000毫米;曝光60毫米）。尽管用MX观察到谷胱甘肽水平呈剂量依赖性下降，但这不一定是观察到的DNA损伤的原因，因为在细胞内谷胱甘肽消耗至马来酸二乙酯至对照的23％时未见链断裂。另外，由于没有观察到MX诱导的钙存储释放，因此链断裂似乎不由细胞内钙的升高介导。然而，链断裂可能是Ca〜（2+）依赖性的，如Quin-2剥夺Ca〜（2+）后的抑制所证明的，氯化富马酸（> 3μg / ml）也耗尽了谷胱甘肽和诱导链长时间暴露（60分钟）时，会以亚细胞毒性剂量（最高300μg / ml）断裂。但是，未氯化的物质同样能够引起DNA链断裂（不消耗谷胱甘肽），因此MX似乎是氯化腐殖质的许多成分中只有一种能够诱导DNA链断裂。

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