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首页> 外文期刊>Mutation Research, C. Mutation Research Letters >INFLUENCE OF DNA SUPERCOILING ON CISPLATIN TOXICITY IN ESCHERICHIA COLI K-12
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INFLUENCE OF DNA SUPERCOILING ON CISPLATIN TOXICITY IN ESCHERICHIA COLI K-12

机译：DNA超螺旋作用对大肠杆菌K-12中CISPLATIN毒性的影响

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摘要

DNA supercoiling is known to modulate the activity of numerous promoters in vitro and in vivo. Moreover, it has been reported to modulate the rate of formation of cisplatin/DNA crosslinks in vitro. In order to address the question of how the topology influences CDDP toxicity in E. coli, three mutants with altered gyrase activity which led to a decrease of about 25% in superhelical density were studied. Mutant strains gyrA(224) and gyrB(225) showed abolished sensitivity to CDDP as the parental strain while the grypB(226) mutant was resistant. This resistant was abolished in uvrA (excision-repair) and recA (recombination and SOS processes) mutant derivatives. Thus supercoiling might play a role as an indirect modulator of CDDP toxicity in bacteria by interfering with repair processes.
机译：已知DNA超螺旋在体外和体内可调节许多启动子的活性。此外，据报道在体外调节顺铂/ DNA交联的形成速率。为了解决拓扑结构如何影响大肠杆菌中CDDP毒性的问题，研究了三种具有回旋酶活性变化的突变体，这些突变体导致超螺旋密度降低了约25％。突变株gyrA（224）和gyrB（225）表现出对CDDP的敏感性消失，而grypB（226）突变株具有抗性。在uvrA（切除修复）和recA（重组和SOS过程）突变体衍生物中消除了这种抗性。因此，超螺旋可能通过干扰修复过程而充当细菌中CDDP毒性的间接调节剂。

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