MUTAGENIC EFFECTS AT HPRT LOCUS AND IN MINISATELLITE SEQUENCES INDUCED IN V79 CELLS BY TREATMENTS WITH UV AND METHYL-NITRO-NITROSO GUANIDINE

Ogheri S; Rampazzo C; Celotti L

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MUTAGENIC EFFECTS AT HPRT LOCUS AND IN MINISATELLITE SEQUENCES INDUCED IN V79 CELLS BY TREATMENTS WITH UV AND METHYL-NITRO-NITROSO GUANIDINE

机译：紫外线和甲基-硝基-亚硝基胍对V79细胞诱发HPRT部位和微型卫星序列的突变效应

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DNA alterations induced in V79 cells treated with UV light or methyl-nitro-nitrosoguanidine were analyzed by the mutagenicity test at the hprt locus and by DNA fingerprint analysis. Treated and control cells were seeded in the presence or absence of 6-thioguanine to determine mutant frequency and cell survival. From clonal cultures of the same cell populations we isolated a number of clones and grew them up individually to obtain appropriate amounts of DNA. High molecular weight DNA was digested with HinfI or HaeIII and hybridized with P-32-labelled 33.15 multilocus probe. The induction of 6-thioguanine resistant cells depended on the mutagen dose. The highest value of mutant frequency obtained was 7475 x 10(-6) (MNNG, 27 mu M), corresponding to 0.7 percent of clonable cells. DNA fingerprint analysis carried out on the same treated cells showed that DNA rearrangements occurred at minisatellites much more frequently than in transcribed sequences. UV irradiation produced the highest frequency of variation, modifying minisatellite patterns in about 50 percent of the analyzed clones.

机译：通过在hprt基因座处的致突变性测试和DNA指纹分析，分析了用紫外线或甲基-硝基-亚硝基胍处理的V79细胞中诱导的DNA改变。在有或没有6-硫鸟嘌呤的情况下播种处理过的和对照细胞，以确定突变体的频率和细胞存活率。从相同细胞群的克隆培养物中，我们分离出许多克隆，并使其分别长大以获得适当数量的DNA。用HinfI或HaeIII消化高分子量DNA，并与P-32标记的33.15多基因座探针杂交。对6-硫代鸟嘌呤抗性细胞的诱导取决于诱变剂量。获得的突变频率的最高值为7475 x 10（-6）（MNNG，27μM），相当于可克隆细胞的0.7％。在相同处理过的细胞上进行的DNA指纹分析表明，与转录序列相比，小卫星上发生DNA重排的频率更高。紫外线照射产生最高的变异频率，在大约50％的分析克隆中修饰了微卫星模式。

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《Mutation Research, C. Mutation Research Letters》 |1995年第4期|共7页
作者
Ogheri S; Rampazzo C; Celotti L;
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正文语种 eng
中图分类遗传学;
关键词
Cell culture; Dna fingerprint analysis; Mutant frequency; Mutagenic; Dna;

机译：细胞培养;Dna指纹分析;突变频率;致突变性;Dna;

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1. MUTAGENIC EFFECTS AT HPRT LOCUS AND IN MINISATELLITE SEQUENCES INDUCED IN V79 CELLS BY TREATMENTS WITH UV AND METHYL-NITRO-NITROSO GUANIDINE [J] . Ogheri S, Rampazzo C, Celotti L Mutation Research, C. Mutation Research Letters . 1995,第4期

机译：紫外线和甲基-硝基-亚硝基胍对V79细胞诱发HPRT部位和微型卫星序列的突变效应
2. MUTANT FREQUENCY AT THE HPRT LOCUS AND IN MINISATELLITE SEQUENCES IN CHINESE HAMSTER V79 CELLS IRRADIATED WITH LOW-ENERGY PROTONS (31 KEV/MU-M) AND ULTRAVIOLET LIGHT (254 NM) [J] . Ogheri S., Cera F., Favaretto S., Radiation Research: Official Organ of the Radiation Research Society . 1997,第3期

机译：低能质子（31 KEV / MU-M）和紫外光（254 NM）照射的中国仓鼠V79细胞的HPRT位置和微型卫星序列的突变频率
3. Mutagenic effects at HPRT locus induced in Chinese hamster ovary cells by thermal neutrons with or without boron compound. [J] . Kinashi Y, Masunaga S, Takagaki M, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 1997,第2期

机译：有或没有硼化合物的热中子在中国仓鼠卵巢细胞中对HPRT基因座的诱变作用。
4. Combined treatment of photodynamic therapy and the topoisomerase I inhibitor camptothecin in growing V79 and NHIK 3025 cells [C] . Jean-Michel Gaullier, National Museum for Natural History, Paris Cedex 05, Photochemotherapy: Photodynamic Therapy and Other Modalities II . 1996

机译：光动力疗法和拓扑异构酶I抑制剂喜树碱在生长中的V79和NHIK 3025细胞中的联合治疗
5. Ribozyme-mediated REV1L inhibition reduces the frequency of UV-induced mutations in the human HPRT gene. [D] . Clark, Denise Renee. 2003

机译：核酶介导的REV1L抑制作用降低了人类HPRT基因中紫外线诱导的突变的频率。
6. Effects of Titanium Dioxide Nanoparticles on the Hprt Gene Mutations in V79 Hamster Cells [O] . Alena Kazimirova, Naouale El Yamani, Laura Rubio, 2020

机译：纳米二氧化钛对V79仓鼠细胞中Hprt基因突变的影响
7. Structural Changes in HPRT Gene of V79 Cells After Irradiation With Heavy Ions—Immediate and Delayed Effects [O] . Pavel Bláha, Igor V. Koshlan, Nataliya A. Koshlan, 2021

机译：辐照与延迟效应照射后V79细胞HPRT基因的结构变化
8. Results of an In Vitro Mammalian Cell Gene Mutation Test (HPRT-locus) in Chinese Hamster V79 Cells with ARALDITE PT 910 (TK 30041). [R] . 2015

机译：用aRaLDITE pT 910（TK 30041）在中国仓鼠V79细胞中进行体外哺乳动物细胞基因突变试验（HpRT-基因座）的结果。

MUTAGENIC EFFECTS AT HPRT LOCUS AND IN MINISATELLITE SEQUENCES INDUCED IN V79 CELLS BY TREATMENTS WITH UV AND METHYL-NITRO-NITROSO GUANIDINE

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