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首页> 外文期刊>Mutation Research, C. Mutation Research Letters >CLASTOGENICITY OF ISOAMYLENE OXIDE TO RAT LYMPHOCYTES IN CULTURE
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CLASTOGENICITY OF ISOAMYLENE OXIDE TO RAT LYMPHOCYTES IN CULTURE

机译：异戊烯氧化物对培养大鼠淋巴细胞的致死作用

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摘要

The mutagenic activity of the aliphatic epoxide isoamylene oxide (2-methyl-2,3-epoxybutane) is not readily detectable in the standard Ames test. In this study, the clastogenic potential of isoamylene oxide was evaluated using an in vitro mammalian cell culture system. Approximately 48 h after establishing primary cultures of rat lymphocyte cultures, the cells were treated for 4 h with various concentrations of isoamylene oxide (50, 166.7, 500, 1666.7, and 5000 mu g/ml in the initial assay and 500, 1000, 2000, 3000, 4000, and 5000 mu g/ml in the confirmatory assay). The cultures were harvested 24 h after termination of the treatment. Based upon the mitotic indices, cultures treated with the three highest concentrations in both the initial and confirmatory assays were evaluated to estimate the chromosomal aberration frequencies. Isoamylene oxide demonstrated a strong clastogenic activity in this assay: up to 29% aberrant cells (without gaps) were observed at the highest concentration analyzed. The presence of an external metabolic activation system (S9) did not seem to influence the magnitude of the response at the dose levels analyzed.
机译：在标准的Ames测试中，脂肪族环氧化物异戊烯（2-甲基-2,3-环氧丁烷）的诱变活性不易检测到。在这项研究中，使用体外哺乳动物细胞培养系统评估了异戊烯的裂解潜力。建立大鼠淋巴细胞培养物的原始培养物后约48小时，在初始测定中分别用各种浓度的异戊烯氧化物（50、166.7、500、1666.7和5000μg / ml）处理细胞4小时，在500、1000、2000中（在确认试验中为3000、4000和5000μg / ml）。处理终止后24小时收获培养物。基于有丝分裂指数，对在初始和验证试验中用三种最高浓度处理的培养物进行评估，以估计染色体畸变频率。氧化异戊烯在该测定法中表现出强大的裂解活性：在最高分析浓度下，观察到高达29％的异常细胞（无间隙）。外部代谢激活系统（S9）的存在似乎并未影响所分析剂量水平下反应的幅度。

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