Comparison of lad and lacZ transgenic mouse mutation assays: an EU-sponsored interlaboratory study

H. Tinwell; U. Liegibel; O. Krebs; P. Schmezer; J. Favor; J. Ashby

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Comparison of lad and lacZ transgenic mouse mutation assays: an EU-sponsored interlaboratory study

机译：lad和lacZ转基因小鼠突变检测方法的比较：一项欧盟资助的实验室间研究

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As part of an EU Environment Programme sponsored study, the performance of two transgenic mouse mutation assays has been evaluated in three laboratories using common liver samples. The systems studied were the lacI~-(Big Blue~(TM)) mutation assay, andthe GalE~- positive selection modified lacZ~ (Muta~(TM)Mouse) assay. The liver samples compared were derived from mice treated with either saline or dimethylnitrosamine (DMN; 14 day recovery). Each assay gave an increased mutation frequency (MF) for theDMN'treated livers when compared to the saline control MFs. Sources of variability in the assays are discussed, and it is concluded that whole liver should be homogenised before DNA extraction, and that concurrent controls should be processed in parallel with test samples.

机译：作为欧盟环境计划资助的研究的一部分，已经在三个实验室中使用普通肝样品评估了两种转基因小鼠突变测定的性能。所研究的系统是lacI-（Big Blue（TM））突变测定法和GalE--正选择修饰的lacZ（（Muta TM）小鼠）测定法。比较的肝样品来自用盐水或二甲基亚硝胺治疗的小鼠（DMN; 14天恢复）。与盐水对照MFs相比，每种测定法给DMN'处理的肝脏增加了突变频率（MF）。讨论了测定方法中变异性的来源，并得出结论，在提取DNA之前，应将整个肝脏均质化，并且应同时处理对照样品和测试样品。

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《Mutation Research, E. Environmental Mutagenesis and Related Subjects》 |1995年第2期|共6页
作者
H. Tinwell; U. Liegibel; O. Krebs; P. Schmezer; J. Favor; J. Ashby;
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正文语种 eng
中图分类遗传学;
关键词
Transgenic mouse mutation assay; LacI; LacZ;

机译：转基因小鼠突变试验;LacI;LacZ;

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专利

1. Comparison of lad and lacZ transgenic mouse mutation assays: an EU-sponsored interlaboratory study [J] . H. Tinwell, U. Liegibel, O. Krebs, Mutation Research, E. Environmental Mutagenesis and Related Subjects . 1995,第2期

机译：lad和lacZ转基因小鼠突变检测方法的比较：一项欧盟资助的实验室间研究
2. Sources of variability in data from a positive selection lacZ transgenic mouse mutation assay: an interlaboratory study. [J] . Piegorsch WW, Lockhart AC, Carr GJ, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 1997,第2a3期

机译：阳性选择lacZ转基因小鼠突变检测的数据变异性来源：实验室间研究。
3. The application of the restriction site mutation assay to compare 1-ethyl-1-nitrosourea-induced mutations between the endogenous p53 gene and the transgenic LacZ gene in MutaMouse testes. [J] . Song HL, Jenkins GJ, Ashby J, Mutagenesis . 2001,第1期

机译：限制性酶切位点突变检测法的应用，以比较1-乙基-1-亚硝基脲诱导的MutaMouse睾丸内源性p53基因与转基因LacZ基因之间的突变。
4. Reproducibility of MRM-based Assays for Quantitative Verification of Candidate Protein Biomarkers in Plasma: an Interlaboratory Study [C] . Terri Addona, Steven C. Hall, Steven J. Skates, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：基于MRM的测定的再现性进行血浆中候选蛋白生物标志物的定量验证：一个间接性研究
5. Characterisation of a conserved motif found within the Dlx cis-regulatory element URE2 and its effect on mouse URE2-lacZ activity in transgenic mice. [D] . Lavoie, Monique V. 2010

机译：在Dlx顺式调控元件URE2中发现的保守基序的特征及其对转基因小鼠中的URE2-lacZ活性的影响。
6. Mutagenicity of the peroxisome proliferators clofibrate Wyeth 14643 and di-2-ethylhexyl phthalate in the lacZ plasmid-based transgenic mouse mutation assay [O] . Michaël ETI Boerrigter 2004

机译：过氧化物酶体增殖物clofibrateWyeth 14643和邻苯二甲酸二-2-乙基己酯在基于lacZ质粒的转基因小鼠突变试验中的致突变性
7. The application of the restriction site mutation assay to compare 1-ethyl-1-nitrosourea-induced mutations between the endogenous p53 gene and the transgenic LacZ gene in MutaMouse testes [O] . H.-L. Song 2001

机译：限制性位点突变测定的应用比较内源P53基因与转基因LacZ基因之间的1-乙基-1-亚核诱导突变在Mutamouse睾丸中的突变
8. Correlation of Carcinogenic Potency with Mouse-Skin (32)P-Postlabeling and Muta-(R)Mouse lac Z(-)Mutation Data for DMBA and Its K-Region Sulphur Isostere: Comparison with Activities Observed in Standard Genotoxicity Assays [R] . Ashby, J., Brusick, D., Myhr, B. C., 1993

机译：致癌效力与小鼠皮肤的相关性（32）p-标记后和muta-（R）小鼠lac Z（ - ）DmBa及其K区硫磺等位基因的突变数据：与标准基因毒性检测中观察到的活性的比较

Comparison of lad and lacZ transgenic mouse mutation assays: an EU-sponsored interlaboratory study

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