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Evaluation of properties of several cheese-ripening fungi for potential biotechnological applications

机译:评价几种干酪发酵真菌的潜在生物技术应用性能

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摘要

Strains of Penicillium camemberti and Penicillium roqueforti were tested for properties that could be important for future biotechnological applications of these fungi. Penicillium camemberti CECT 2267 and P. roqueforti NRRL 849 showed the most promising performances in terms of growth, protoplast production, and protoplast regeneration abilities. Transformation of these strains with a plasmid containing gene encoding phleomycin resistance showed that they also have a high transformation frequency. In addition, both strains showed low extracellular proteolytic activity. Thus, these strains have all the characteristics to make them suitable for future genetic improvement, recombinant protein production, and other potential biotechnological applications.
机译:测试了卡门培尔青霉和罗氏青霉菌株的特性,这些特性对于这些真菌的未来生物技术应用而言可能是重要的。在生长,原生质体产生和原生质体再生能力方面,卡门培尔青霉CECT 2267和罗克福尔霉NRRL 849表现出最有希望的表现。用含有编码毛霉素抗性的基因的质粒转化这些菌株表明它们也具有高转化频率。另外,两种菌株均显示出低的细胞外蛋白水解活性。因此,这些菌株具有使其适合于将来的遗传改良,重组蛋白生产以及其他潜在生物技术应用的所有特征。

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