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首页> 外文期刊>Mutation research. Genetic toxicology testing >CHROMOSOME ABERRATION TEST FOR THE INSECTICIDE, DICHLORVOS, ON FISH CHROMOSOMES
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CHROMOSOME ABERRATION TEST FOR THE INSECTICIDE, DICHLORVOS, ON FISH CHROMOSOMES

机译：鱼染色体上杀虫剂敌敌畏的染色体畸变试验

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Chromosome aberration test was applied for an organophosphorus insecticide, dichlorvos, using fish as model. Channa punctatus, an ophiocephalid, having a small number (2n = 32) and a rather large size of chromosomes, was used for the purpose. Dichlorvos, with O,O-dimethyl-2,2-dichlorovinyl phosphate as the active component, was dissolved in the aquarium water at concentration level equal to that found in drainage from agricultural fields (0.01 ppm). The chromosomal preparations were made from the kidney cells of the fish after 24, 48, 72 and 96 h intervals. Controls were kept in ordinary water. The aberrations observed were chromatid gaps, sub-chromatid gaps, centromeric gaps, precocious separation of chromatids and polyploidy. These were found to be significantly higher as compared to that of the controls. This test proved its efficacy in the fish model without the administration of the chemical by injection.
机译：以鱼为模型，对有机磷杀虫剂敌敌畏进行了染色体畸变试验。为此目的，使用了少量（2n = 32）和相当大的染色体大小的点状突鼻。以O，O-二甲基-2,2-二氯乙烯基磷酸酯为活性成分的敌敌畏以与农田排水相同的浓度水平（0.01 ppm）溶解在水族馆水中。在间隔24、48、72和96小时后，由鱼的肾细胞制成染色体制剂。对照保存在普通水中。观察到的像差是染色单体间隙，亚染色单体间隙，着丝粒间隙，早熟的染色单体分离和多倍性。发现这些与对照相比显着更高。该测试证明了它在鱼模型中的有效性，而无需通过注射方式施用化学药品。

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