...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular Breeding >Development and integration of EST-SSR markers into an established linkage map in switchgrass
【24h】

Development and integration of EST-SSR markers into an established linkage map in switchgrass

机译：EST-SSR标记的开发和整合到柳枝established中已建立的连锁图中

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Switchgrass (Panicum virgatum L.) is a model cellulosic biofuel crop in the United States. Simple sequence repeat (SSR) markers are valuable resources for genetic mapping and molecular breeding. A large number of expressed sequence tags (ESTs) of switchgrass are recently available in our sequencing project. The objectives of this study were to develop new SSR markers from the switchgrass EST sequences and to integrate them into an existing linkage map. More than 750 unique primer pairs (PPs) were designed from 243,600 EST contigs and tested for PCR amplifications, resulting in 538 PPs effectively producing amplicons of expected sizes. Of the effective PPs, 481 amplifying informative bands in NL94 were screened for polymorphisms in a panel consisting of NL94 and its seven first-generation selfed (S1) progeny. This led to the selection of 117 polymorphic EST-SSRs to genotype a mapping population encompassing 139 S1 individuals of NL94. Of 83 markers demonstrating clearly scorable alleles in the mapping population, 79 were integrated into a published linkage map, with three linked to accessory loci and one unlinked. The newly identified EST-SSR loci were distributed in 17 of 18 linkage groups with 27 (32.5 %) exhibiting distorted segregations. The integration of EST-SSRs aided in reducing the average marker interval (cM) to 3.7 from 4.2, and reduced the number of gaps (each > 15 cM) to 10 from 23. Developing new EST-SSRs and constructing a higher density linkage map will facilitate quantitative trait locus mapping and provide a firm footing for marker-assisted breeding in switchgrass.
机译：柳枝（（Panicum virgatum L.）是美国的一种典型的纤维素生物燃料作物。简单序列重复（SSR）标记是用于遗传作图和分子育种的宝贵资源。最近在我们的测序项目中可获得大量的柳枝switch表达序列标签（EST）。这项研究的目的是从柳枝EST序列开发新的SSR标记，并将其整合到现有的连锁图中。从243,600个EST重叠群中设计了750多个独特的引物对（PP），并对其进行PCR扩增测试，结果得到538个PP有效地产生了预期大小的扩增子。在有效的PP中，在NL94及其七个第一代自交（S1）后代组成的组中，筛选了NL94中481个扩增信息带的多态性。这导致选择了117个多态EST-SSR以对包括139个NL1 S1个体的作图群体进行基因分型。在作图人群中明显可评分的等位基因的83个标记中，有79个被整合到已发布的连锁图中，其中三个与辅助基因座连锁，一个未连锁。新近确定的EST-SSR基因座分布在18个连锁组中的17个中，其中27个（32.5％）表现出扭曲的分离。 EST-SSR的整合有助于将平均标记间隔（cM）从4.2降低到3.7，并将缺口的数量（每个> 15 cM）从23减少到10。开发新的EST-SSR和构建更高的密度连锁图将有助于定量性状基因座作图，并为柳枝switch标记辅助育种提供坚实的基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号