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首页> 外文期刊>Mutation research. Genetic toxicology testing >ANALYSIS OF MUTAGENESIS IN UV-SENSITIVE MOUSE LYMPHOMA L5178Y-R CELLS WITH A POLYOMAVIRUS-BASED SHUTTLE VECTOR
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ANALYSIS OF MUTAGENESIS IN UV-SENSITIVE MOUSE LYMPHOMA L5178Y-R CELLS WITH A POLYOMAVIRUS-BASED SHUTTLE VECTOR

机译：带有多聚微球蛋白的穿梭载体的紫外线敏感小鼠淋巴瘤L5178Y-R细胞的诱变分析

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摘要

A newly-developed polyomavirus-based shuttle vector, pPySLPT-2, was used to analyze mutations induced by UV radiation in the two related mouse lymphoma cell lines, L5178Y-R (LY-R) and L5178Y-S (LY-S). These well-studied cell lines differ in sensitivity to UV radiation, apparently due to differences in DNA repair capacity. Consistent with these differences, we found that replication of UV-irradiated vector is inhibited to a greater extent in the UV sensitive LY-R cells than in the LY-S cells. Mutations were induced in the vector's supF target gene at a higher frequency in the LY-R cells than in the LY-S cells, but the sequence characteristics of the base substitution mutations were similar in the two cell lines. The pattern of UV-induced mutations in the supF target gene of this polyomavirus-based vector, replicated in the mouse cells, was very similar to the pattern observed in the same target gene in the analogous simian virus 40-based vector, pZ189, replicated in monkey and human cells.
机译：一种新开发的基于多瘤病毒的穿梭载体pPySLPT-2，用于分析两种相关小鼠淋巴瘤细胞系L5178Y-R（LY-R）和L5178Y-S（LY-S）中由紫外线辐射诱导的突变。这些经过充分研究的细胞系对紫外线辐射的敏感性不同，这显然是由于DNA修复能力的差异。与这些差异一致，我们发现在紫外线敏感的LY-R细胞中，比在LY-S细胞中，紫外线辐射载体的复制受到更大程度的抑制。在载体的supF靶基因中，LY-R细胞中的诱变频率高于LY-S细胞中的诱变频率，但两种细胞系中碱基取代突变的序列特征相似。在小鼠细胞中复制的这种基于多瘤病毒的载体的supF目标基因中的紫外线诱导的突变模式与在类似猿猴病毒40的载体pZ189中复制的同一目标基因中观察到的模式非常相似在猴子和人类细胞中。

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