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首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Broad spectrum antimutagenic activity of antioxidant active fraction of punica granatum L. peel extracts.
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Broad spectrum antimutagenic activity of antioxidant active fraction of punica granatum L. peel extracts.

机译：石榴皮提取物抗氧化活性成分的广谱抗突变活性。

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摘要

Over the past few decades, scientific research has indicated a credible basis for some of the traditional ethnomedicinal uses of pomegranate. This study aims to evaluate the broad spectrum antioxidant and antimutagenic activities of peel extracts of pomegranate. The sequentially extracted Punica granatum peel fractions were tested for their antioxidant activity by DPPH free radical scavenging, phosphomolybdenum, FRAP (Fe(3+) reducing power) and CUPRAC (cupric ions (Cu(2+)) reducing ability) assays. The methanol fraction showed highest antioxidant activity by all the four in vitro assays comparable to ascorbic acid and butylated hydroxy toluene (BHT) followed by activity in ethanol, acetone, and ethyl acetate fractions. Based on the promising antioxidant activities, the methanol fraction was evaluated for antimutagenic activity by Ames Salmonella/microsome assay against sodium azide (NaN(3)), methyl methane sulphonate (MMS), 2-aminofluorene (2-AF) and benzo(a)pyrene (B(a)P) induced mutagenicity in Salmonella typhimurium (TA97a, TA98, TA100 and TA102) tester strains. The methanol fraction showed no sign of mutagenicity at tested concentration of 10-80mug/mL. This fraction showed antimutagenic activity against NaN(3) and MMS with percent inhibition of mutagenicity ranging from 66.76% to 91.86% in a concentration-dependent manner. Similar trend of inhibition of mutagenicity (81.2-88.58%) against indirect mutagens (2-AF and B(a)P) was also recorded. Phytochemical analysis by HPLC, LC-MS and total phenolic content revealed high content of ellagitannins which might be responsible for promising antioxidant and antimutagenic activities of P. granatum peel extract. Further, contribution of bioactive compounds detected in this study is to be explored to understand the exact mechanism of action as well as their therapeutic efficacy.
机译：在过去的几十年中，科学研究已为石榴的某些传统民族医学用途提供了可靠的基础。本研究旨在评估石榴皮提取物的广谱抗氧化和抗诱变活性。通过DPPH自由基清除，磷钼，FRAP（Fe（3+）还原能力）和CUPRAC（铜离子（Cu（2+））还原能力）试验测试了顺序提取的石榴皮部分的抗氧化活性。与抗坏血酸和丁基化羟基甲苯（BHT）相比，在所有四个体外测定中，甲醇馏分均显示出最高的抗氧化活性，其后在乙醇，丙酮和乙酸乙酯馏分中具有活性。基于有希望的抗氧化活性，通过Ames沙门氏菌/微粒体测定法评估了甲醇馏分对叠氮化钠（NaN（3）），甲烷磺酸甲酯（MMS），2-氨基芴（2-AF）和苯并（a）的抗诱变活性。 py（B（a）P）在鼠伤寒沙门氏菌（TA97a，TA98，TA100和TA102）测试菌株中诱导诱变。在测试浓度为10-80 ug / mL时，甲醇馏分未显示出致突变性。该级分显示出对NaN（3）和MMS的抗诱变活性，致突变率的百分比抑制范围为66.76％至91.86％，呈浓度依赖性。还记录了对间接诱变剂（2-AF和B（a）P）的致突变性抑制作用（81.2-88.58％）的相似趋势。 HPLC，LC-MS和总酚含量的植物化学分析显示，鞣花单宁含量高，这可能是有希望的颗粒状果皮提取物的抗氧化和抗突变活性。此外，将探索本研究中检测到的生物活性化合物的作用，以了解确切的作用机理及其治疗功效。

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