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首页> 外文期刊>Molecular cell >A Cell Cycle-Regulated GATA Factor Promotes Centromeric Localization of CENP-A in Fission Yeast.
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A Cell Cycle-Regulated GATA Factor Promotes Centromeric Localization of CENP-A in Fission Yeast.

机译：细胞周期调节的GATA因子促进裂变酵母中CENP-A的着丝粒定位。

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摘要

CENP-A, the centromere-specific histone H3 variant, plays a crucial role in organizing kinetochore chromatin for precise chromosome segregation. We have isolated Ams2, a Daxx-like motif-containing GATA factor, and histone H4, as multicopy suppressors of cnp1-1, an S. pombe CENP-A mutant. While depletion of Ams2 results in the reduction of CENP-A binding to the centromere and chromosome missegregation, increasing its dosage restores association of a CENP-A mutant protein with centromeres. Conversely, overexpression of CENP-A or histone H4 suppresses an ams2 disruptant. The intracellular amount of Ams2 thus affects centromeric nucleosomal constituents. Ams2 is abundant in S phase and associates with chromatin, including the central centromeres through binding to GATA-core sequences. Ams2 is thus a cell cycle-regulated GATA factor that is required for centromere function.
机译：CENP-A，着丝粒特异的组蛋白H3变体，在组织线粒体染色质以精确分离染色体中起着至关重要的作用。我们已经分离出Ams2，一个Daxx样基序包含GATA因子和组蛋白H4，作为粟酒裂殖酵母CENP-A突变体cnp1-1的多拷贝抑制剂。尽管Ams2的消耗导致CENP-A与着丝粒的结合减少和染色体错聚，但增加其剂量可恢复CENP-A突变蛋白与着丝粒的关联。相反，CENP-A或组蛋白H4的过表达抑制了ams2破坏剂。因此，Ams2的细胞内量会影响着丝粒核小体成分。 Ams2在S期丰富，并通过结合GATA核心序列与染色质（包括中央着丝粒）缔合。因此，Ams2是着丝粒功能所需的细胞周期调节的GATA因子。

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