The Response Regulator BvgA and RNA Polymerase alpha Subunit C-Terminal Domain Bind Simultaneously to Different Faces of the Same Segment of Promoter DNA.

Boucher PE; Maris AE; Yang MS; Stibitz S

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The Response Regulator BvgA and RNA Polymerase alpha Subunit C-Terminal Domain Bind Simultaneously to Different Faces of the Same Segment of Promoter DNA.

机译：响应调节剂BvgA和RNA聚合酶α亚基C末端域同时绑定到启动子DNA同一段的不同面孔。

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摘要

Examination of the binding of FeBABE-conjugated BvgA to the fha promoter of Bordetella pertussis has revealed that three dimers, formed by head-to-head association of monomers, bind one face of the DNA helix from the inverted-heptad primary binding site to the -35 region. The orientation of BvgA monomers within the dimers is the same as that recently demonstrated by X-ray crystallographic methods for a dimer of the C-terminal domain of NarL bound to DNA. Use of FeBABE conjugates of RNAP alpha subunit C-terminal domain showed that binding of this domain is linearly coincident with binding of the BvgA dimers, but to a different helical face. These results reveal a previously undescribed mode of interaction between RNAP alpha-CTD and a transcriptional activator.

机译：FeBABE缀合的BvgA与百日咳博德特氏菌的fha启动子的结合研究表明，由单体的头对头结合形成的三个二聚体将DNA螺旋的一个面从倒七联的初级结合位点结合到了螺旋藻上。 -35地区。 BvgA单体在二聚体中的取向与最近通过X射线晶体学方法证明与DNA结合的NarL C端结构域的二聚体的取向相同。使用RNA聚合酶α亚基C末端结构域的FeBABE共轭物显示，该结构域的结合与BvgA二聚体的结合线性一致，但结合到不同的螺旋面上。这些结果揭示了RNAPα-CTD和转录激活因子之间以前未描述的相互作用方式。

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《Molecular cell》 |2003年第1期|共11页
作者
Boucher PE; Maris AE; Yang MS; Stibitz S;
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正文语种 eng
中图分类分子生物学;细胞生物学;
关键词
Carbon ion; Face; DNA; binding; FeBABE; 面部;

机译：Carbon ion;Face;DNA;binding;FeBABE;面部;

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1. The Response Regulator BvgA and RNA Polymerase alpha Subunit C-Terminal Domain Bind Simultaneously to Different Faces of the Same Segment of Promoter DNA. [J] . Boucher PE, Maris AE, Yang MS, Molecular cell . 2003,第1期

机译：响应调节剂BvgA和RNA聚合酶α亚基C末端域同时绑定到启动子DNA同一段的不同面孔。
2. Mutations Affecting the α Subunit ofBordetella pertussis RNA Polymerase Suppress Growth Inhibition Conferred by Short C-Terminal Deletions of the Response Regulator BvgA [J] . Scott Stibitz Journal of bacteriology . 1998,第9期

机译：影响百日咳博德特氏菌RNA聚合酶α亚基的突变抑制应答调节因子BvgA的短C末端缺失所导致的生长抑制。
3. Interactions between the Escherichia coli cAMP receptor protein and the C-terminal domain of the alpha subunit of RNA polymerase at class I promoters. [J] . Law EC, Savery NJ, Busby SJW The Biochemical Journal . 1999,第Pta3期

机译：大肠杆菌cAMP受体蛋白与I类启动子上RNA聚合酶α亚基的C末端结构域之间的相互作用。
4. Analysis of promoter binding proteins of Ebp1 that is inhibitor protein of influenza virus RNA polymerase [C] . Mukai M., Honda A. Micro-NanoMechatronics and Human Science, 2009. MHS 2009 . 2009

机译：流行性感冒病毒RNA聚合酶抑制剂蛋白Ebp1的启动子结合蛋白分析
5. Binding of C-terminal domain to the core domain of T4 gene 32 protein: Competition with single-stranded DNA. [D] . Anderson, Brian. 2010

机译：C末端结构域与T4基因32蛋白的核心结构域的结合：与单链DNA的竞争。
6. Note: Transcriptional Activation of the Bacillus subtilis spoIIG Promoter by the Response Regulator Spo0A Is Independent of the C-Terminal Domain of the RNA Polymerase Alpha Subunit [O] . Dean A. Rowe-Magnus, Mario Mencía, Fernando Rojo, 1998

机译：注意：响应调节剂Spo0A枯草芽孢杆菌spoIIG启动子的转录激活独立于RNA聚合酶α亚基的C末端域。
7. Mutations Affecting the α Subunit of Bordetella pertussis RNA Polymerase Suppress Growth Inhibition Conferred by Short C-Terminal Deletions of the Response Regulator BvgA [O] . Scott Stibitz 1998

机译：影响Bordetella Pertussis的α亚基的突变抑制响应调节剂BVGA的短C末端缺失赋予的生长抑制

The Response Regulator BvgA and RNA Polymerase alpha Subunit C-Terminal Domain Bind Simultaneously to Different Faces of the Same Segment of Promoter DNA.

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