A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.

Sullivan KD; Steiniger M; Marzluff WF

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A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.

机译：CPSF73，CPSF100和Symplekin的核心复合物可能形成两个不同的切割因子，用于加工poly（A）和组蛋白mRNA。

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Metazoan histone mRNAs are unique: their pre-mRNAs contain no introns, and the mRNAs are not polyadenylated, ending instead in a conserved stem-loop structure. In Drosophila, canonical poly(A) signals are located downstream of the normal cleavage site of each histone gene and are utilized when histone 3' end formation is inhibited. Here we define a subcomplex of poly(A) factors that are required for histone pre-mRNA processing. We demonstrate that Symplekin, CPSF73, and CPSF100 are present in a stable complex and interact with histone-specific processing factors. We use chromatin immunoprecipitation to show that Symplekin and CPSF73, but not CstF50, cotranscriptionally associate with histone genes. Depletion of SLBP recruits CstF50 to histone genes. Knockdown of CPSF160 or CstF64 downregulates Symplekin but does not affect histone pre-mRNA processing or association of Symplekin with the histone locus. These results suggest that a common core cleavage factor is required for processing of histone and polyadenylated pre-mRNAs.

机译：后生动物组蛋白的mRNA是独特的：它们的前mRNA不包含内含子，并且mRNA没有聚腺苷酸化，而是以保守的茎环结构终止。在果蝇中，规范化的poly（A）信号位于每个组蛋白基因正常切割位点的下游，并在抑制组蛋白3'末端形成时使用。在这里，我们定义了组蛋白前mRNA处理所需的poly（A）因子的亚复合物。我们证明了Symplekin，CPSF73和CPSF100存在于稳定的复合物中，并与组蛋白特异性加工因子相互作用。我们使用染色质免疫沉淀来显示Symplekin和CPSF73，但不是CstF50，与组蛋白基因共转录结合。 SLBP耗竭将CstF50募集到组蛋白基因上。敲低CPSF160或CstF64会下调Symplekin，但不影响组蛋白前mRNA加工或Symplekin与组蛋白基因座的关联。这些结果表明，加工组蛋白和聚腺苷酸化的pre-mRNA需要一个共同的核心切割因子。

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《Molecular cell》 |2009年第3期|共11页
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Sullivan KD; Steiniger M; Marzluff WF;
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中图分类分子生物学;细胞生物学;
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1. A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs. [J] . Sullivan KD, Steiniger M, Marzluff WF Molecular cell . 2009,第3期

机译：CPSF73，CPSF100和Symplekin的核心复合物可能形成两个不同的切割因子，用于加工poly（A）和组蛋白mRNA。
2. Systematic Profiling of Poly(A)+ Transcripts Modulated by Core 3’ End Processing and Splicing Factors Reveals Regulatory Rules of Alternative Cleavage and Polyadenylation [J] . Wencheng Li, Bei You, Mainul Hoque, PLoS Genetics . 2015,第4期

机译：核心3'末端加工和剪接因子调节的Poly（A）+转录本的系统分析揭示了选择性切割和聚腺苷酸化的调控规则。
3. The stem-loop binding protein forms a highly stable and specific complex with the 3' stem-loop of histone mRNAs. [J] . Battle DJ, Doudna JA RNA . 2001,第1期

机译：茎环结合蛋白与组蛋白mRNA的3'茎环形成高度稳定且特异性的复合物。
4. Effect of acrylic core-shell impact modifier on processing and thermo-mechanical properties of stereocomplex poly(lactic acid) [C] . Jacek Leciński, Andrea Siebert-Raths, Daniela Jahn Technical Conference of the Society of Plastics Engineers . 2017

机译：丙烯酸核 - 壳体抗冲改性剂对立体复合物聚（乳酸）加工及热力学性能的影响
5. Functional interaction between PIPKIalpha and the non-canonical poly(A) polymerase star-PAP for the efficient 3' end formation of select mRNAs. [D] . Mellman, David Lorenz. 2009

机译：PIPKIalpha和非经典的poly（A）聚合酶star-PAP之间的功能相互作用，可实现选定mRNA的有效3'端形成。
6. A core complex of CPSF73 CPSF100 and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs [O] . Kelly D. Sullivan, Mindy Steiniger, William F. Marzluff -1

机译：复杂CpsF73CpsF100和symplekin的芯可以形成两个不同切割因子多聚（a）和组蛋白的mRNa的加工
7. A Core Complex of CPSF73, CPSF100, and Symplekin May Form Two Different Cleavage Factors for Processing of Poly(A) and Histone mRNAs [O] . Kelly D. Sullivan, Mindy Steiniger, William F. Marzluff 2009

机译：CPSF73，CPSF100和Symplekin的核心复合物可以形成两个不同的裂解因子，用于加工聚（A）和组蛋白MRNA

A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.

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