TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II.

Akhtar MS; Heidemann M; Tietjen JR; Zhang DW; Chapman RD; Eick D; Ansari AZ

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TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II.

机译：TFIIH激酶在RNA聚合酶II的羧基末端结构域上放置了二价标记。

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Posttranslational modifications of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) specify a molecular recognition code that is deciphered by proteins involved in RNA biogenesis. The CTD is comprised of a repeating heptapeptide (Y(1)S(2)P(3)T(4)S(5)P(6)S(7)). Recently, phosphorylation of serine 7 was shown to be important for cotranscriptional processing of two snRNAs in mammalian cells. Here we report that Kin28/Cdk7, a subunit of the evolutionarily conserved TFIIH complex, is a Ser7 kinase. The ability of Kin28/Cdk7 to phosphorylate Ser7 is particularly surprising because this kinase functions at promoters of protein-coding genes, rather than being restricted to promoter-distal regions of snRNA genes. Kin28/Cdk7 is also known to phosphorylate Ser5 residues of the CTD at gene promoters. Taken together, our results implicate the TFIIH kinase in placing bivalent Ser5 and Ser7 marks early in gene transcription. These bivalent CTD marks, in concert with cues within nascent transcripts, specify the cotranscriptional engagement of the relevant RNA processing machinery.

机译：RNA聚合酶II（Pol II）最大亚基的羧基末端结构域（CTD）的翻译后修饰指定了一种分子识别码，该分子识别码由参与RNA生物发生的蛋白质破译。 CTD由重复的七肽（Y（1）S（2）P（3）T（4）S（5）P（6）S（7）组成。最近，丝氨酸7的磷酸化被证明对于哺乳动物细胞中两个snRNA的共转录加工非常重要。在这里我们报告说，Kin28 / Cdk7是进化保守的TFIIH复合体的亚基，是Ser7激酶。 Kin28 / Cdk7磷酸化Ser7的能力尤其令人惊讶，因为该激酶在蛋白质编码基因的启动子上起作用，而不是局限于snRNA基因的启动子-远端区域。还已知Kin28 / Cdk7在基因启动子处使CTD的Ser5残基磷酸化。两者合计，我们的结果暗示TFIIH激酶在基因转录的早期放置二价Ser5和Ser7标记。这些二价CTD标记与新生转录本中的线索共同指定了相关RNA处理机器的共转录参与。

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《Molecular cell》 |2009年第3期|共7页
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Akhtar MS; Heidemann M; Tietjen JR; Zhang DW; Chapman RD; Eick D; Ansari AZ;
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中图分类分子生物学;细胞生物学;
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1. TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II. [J] . Akhtar MS, Heidemann M, Tietjen JR, Molecular cell . 2009,第3期

机译：TFIIH激酶在RNA聚合酶II的羧基末端结构域上放置了二价标记。
2. hnRNP U Inhibits Carboxy-Terminal Domain Phosphorylation by TFIIH and Represses RNA Polymerase II Elongation [J] . Myung K. Kim, Vera M. Nikodem Molecular and Cellular Biology . 1999,第10期

机译：hnRNP U抑制TFIIH修饰羧基末端域的磷酸化并抑制RNA聚合酶II的延伸
3. Carboxy-terminal region of the yeast heat shock factor contains two domains that make transcription independent of the TFIIH protein kinase. [J] . Sakurai H, Hashikawa N, Imazu H, Genes to cells : . 2003,第12期

机译：酵母热激因子的羧基末端区域包含两个域，使转录独立于TFIIH蛋白激酶。
4. MARK, a Maize Atypical Receptor-like Kinase, Expressed During Embryogenesis and in the Meristems of the Adult Plant, interacts with MIK, a New GCK/SPS1 Kinase [C] . BLANCA LLOMPART, ADOLFO RIO, ALEJANDRO FERRANDO, International Workshop on Seeds . 2003

机译：Mark，一种玉米非典型受体样激酶，在胚胎发生期间表达和成人植物的分页，与MIK，一种新的GCK / SPS1激酶相互作用
5. Protein phosphatase-1 dephosphorylates the C-terminal domain of RNA polymerase II. [D] . Washington, Kareem N. 2005

机译：蛋白磷酸酶-1使RNA聚合酶II的C末端结构域去磷酸化。
6. TFIIH kinase places bivalent marks on the carboxyl-terminal domain of RNA polymerase II [O] . M. Sohail Akhtar, Martin Heidemann, Joshua Tietjen, -1

机译：TFIIH激酶地方对RNa的羧基端结构域的二价标记聚合酶II
7. Kin28, the TFIIH-Associated Carboxy-Terminal Domain Kinase, Facilitates the Recruitment of mRNA Processing Machinery to RNA Polymerase II [O] . Rodriguez, Christine R., Cho, Eun-Jung, Keogh, Michael-C., 2000

机译：Kin28，与TFIIH相关的羧基末端结构域激酶，有助于将RNA加工机器招募至RNA聚合酶II

TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II.

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