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首页> 外文期刊>Molecular cell >Involvement of Actin-Related Proteins in ATP-Dependent Chromatin Remodeling
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Involvement of Actin-Related Proteins in ATP-Dependent Chromatin Remodeling

机译：肌动蛋白相关蛋白参与ATP依赖的染色质重塑

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Actin-related proteins (Arps) and conventional actin are enigmatic components of many chromatin-remodeling enzyme complexes. The yeast INO80 ATP-dependent chromatin-remodeling complex contains stoichiometric amounts of Arp4, Arp5, Arp8, and actin. Here we have revealed functions of Arp5 and Arp8 by analysis of mutants. arp5Δ and arp8Δ mutants display an ino80Δ phenotype. Purification of INO80 complexes from arp5Δ and arp8Δ cells shows that protein complexes remain intact but are compromised for INO80 ATPase activity, DNA binding, and nucleosome mobilization. The INO80 (arp8Δ) complex is strikingly deficient, not only for the Arp8 subunit, but also for Arp4 and actin, suggesting an ordered assembly of Arps. Binding of Arp8 to the INO80 complex requires an N-terminal region of Ino80 adjacent to the conserved ATPase domain. GST-Arp8 binds preferentially to histones H3 and H4 in vitro, suggesting a histone chaperone function. These findings show direct involvement of Arps in the chromatin-remodeling process.
机译：肌动蛋白相关蛋白（Arps）和常规肌动蛋白是许多染色质重塑酶复合物的神秘成分。酵母INO80 ATP依赖性染色质重塑复合物含有化学计量的Arp4，Arp5，Arp8和肌动蛋白。在这里，我们通过突变体分析揭示了Arp5和Arp8的功能。 arp5Δ和arp8Δ突变体表现出ino80Δ表型。从arp5Δ和arp8Δ细胞中纯化INO80复合物表明蛋白质复合物保持完整，但因INO80 ATPase活性，DNA结合和核小体动员而受损。 INO80（arp8Δ）复合物非常缺乏，不仅对于Arp8亚基，而且对于Arp4和肌动蛋白，都表明Arps有序组装。 Arp8与INO80复合物的结合需要Ino80的N末端区域与保守的ATPase结构域相邻。 GST-Arp8在体外优先结合组蛋白H3和H4，表明组蛋白伴侣功能。这些发现表明Arps直接参与染色质重塑过程。

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