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首页> 外文期刊>Molecular cell >Recruitment of the Recombinational Repair Machinery to a DNA Double-Strand Break in Yeast
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Recruitment of the Recombinational Repair Machinery to a DNA Double-Strand Break in Yeast

机译：酵母重组DNA双链断裂的重组修复机制的招聘。

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摘要

Repair of DNA double-strand breaks (DSBs) by homologous recombination requires members of the RAD52 epistasis group. Here we use chromatin immunoprecipitation (ChlP) to examine the temporal order of recruitment of Rad51p, Rad52p, Rad54p, Rad55p, and PRA to a single, induced DSB in yeast. Our results suggest a sequential, interdependent assembly of Rad proteins adjacent to the DSB initiated by binding of Rad51p. ChlP time courses from various mutant strains and additional biochemical studies suggest that Rad52p, Rad55p, and Rad54p each help promote the formation and/or stabilization of the Rad51p nucleoprotein filament. We also find that all four Rad proteins associate with homologous donor sequences during strand invasion. These studies provide a near comprehensive view of the molecular events required for the in vivo assembly of a functional Rad51p presynaptic filament.
机译：通过同源重组修复DNA双链断裂（DSB）需要RAD52上位性组的成员。在这里，我们使用染色质免疫沉淀（ChlP）来检查Rad51p，Rad52p，Rad54p，Rad55p和PRA募集到酵母中单个诱导的DSB的时间顺序。我们的结果表明，通过与Rad51p的结合，邻近DSB的Rad蛋白发生了顺序，相互依存的组装。来自各种突变菌株的ChlP时间过程以及其他生化研究表明，Rad52p，Rad55p和Rad54p各自有助于促进Rad51p核蛋白丝的形成和/或稳定。我们还发现，在链入侵期间，所有四种Rad蛋白均与同源供体序列相关。这些研究为功能性Rad51p突触前细丝的体内组装所需的分子事件提供了近乎全面的观点。

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