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The Neurospora crassa chs3 gene encodes an essential class I chitin synthase

机译:Neurospora crassa chs3基因编码必不可少的I类几丁质合酶

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摘要

In Neurospora crassa, at least five different DNA fragments related to chitin synthases have been identified. In this study we have cloned, sequenced, mapped and characterized the chitin synthase 3 structural gene (designated chs3) of N. crassa. Based on the amino acid sequence deduced from the cloned chs3 genomic DNA fragments we have designated this gene as a class I chitin synthase. The chs3 gene is transcribed predominantly at the early stages of mycelial growth and is expressed during all stages of asexual growth. The fact that strains with nuclei harboring a disrupted chs3 gene could only be maintained as heterokaryons indicates that this gene is indispensable in N. crassa. [References: 36]
机译:在神经孢菌中,已鉴定出至少五种与几丁质合酶相关的不同DNA片段。在这项研究中,我们已经克隆,测序,定位和鉴定了猪笼草的几丁质合酶3结构基因(命名为chs3)。基于从克隆的chs3基因组DNA片段推导的氨基酸序列,我们将该基因指定为I类几丁质合酶。 chs3基因主要在菌丝生长的早期阶段转录,并在无性繁殖的所有阶段表达。带有被破坏的chs3基因的核菌株只能以异核体的形式维持这一事实表明,该基因在猪笼草中是必不可少的。 [参考:36]

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