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首页> 外文期刊>Mycologia >Vegetative compatibility between and within Epichloe species.
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Vegetative compatibility between and within Epichloe species.

机译：Epichloe物种之间和内部的营养相容性。

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Epichloe species (Clavicipitaceae, Ascomycota) are biotrophic symbionts of cool-season grasses and grow slowly on defined media. Nine Epichloe biological species (mating populations) have been identified. Vegetative compatibility within or between Epichloe species has not yet been investigated. Chlorate-resistant strains that were nitrate non-utilizing (nit) mutants from 10 isolates representing 4 biological species were selected. Each mutant grew appressed on a defined medium with nitrate as the sole nitrogen source, in contrast to the abundant aerial mycelia produced by the prototrophic wild type isolates on the same medium. Each mutant was putatively identified as nit1 (nitrate reductase structural gene mutant), nit3 (mutant in the pathway-specific regulatory locus or nitrite reductase gene) or NitM (mutant affecting molybdenum cofactor production) by growth phenotypes on medium with nitrite, hypoxanthine, uric acid or ammonium as the sole nitrogen source. When appropriate nit mutants were paired, heterokaryon formation and complementation were indicated by a zone of prototrophic growth. Complementation occurred between complementary mutants of most isolates, even from different species; however, complementation did not occur in all replications. Interspecific heterokaryon formation was confirmed by recovery of parental phenotypes and cosegregation of beta-tubulin gene polymorphisms among single-conidiospore isolates from the complementation zone. There was no indication that mating type interactions affected heterokaryon formation and no discrete vegetative compatibility groups were identified.
机译：毛科物种（虎皮科，子囊菌）是凉季草的生物营养共生体，并在特定的培养基上缓慢生长。现已鉴定出9种Epichloe生物物种（交配种群）。 Epichloe物种内部或之间的营养相容性尚未研究。从代表4个生物物种的10个分离株中筛选出了耐硝酸盐的菌株，它们是硝酸盐未利用（nit）突变体。每个突变体都生长在限定的培养基上，硝酸盐是唯一的氮源，这与原养型野生型分离物在同一培养基上产生的大量气生菌丝形成对比。通过在亚硝酸盐，次黄嘌呤，尿酸培养基上的生长表型，将每个突变体推定为nit1（硝酸盐还原酶结构基因突变体），nit3（途径特异性调控位点或亚硝酸盐还原酶基因中的突变体）或NitM（影响钼辅因子产生的突变体）。酸或铵作为唯一的氮源。当适当的nit突变体配对时，异养核的形成和互补由原养型生长区指示。大多数分离物，甚至来自不同物种的互补突变体之间都发生了互补。但是，并不是所有复制都发生互补。种间异核体的形成通过亲本表型的恢复和β-微管蛋白基因多态性在互补区单生子孢子分离株之间的共分离而得到证实。没有迹象表明交配类型的相互作用会影响异核体的形成，也没有发现离散的营养相容性群体。

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