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首页> 外文期刊>Molecular reproduction and development >Influence of oocyte collection technique on initial chromatin configuration, meiotic competence, and male pronucleus formation after intracytoplasmic sperm injection (ICSI) of equine oocytes.
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Influence of oocyte collection technique on initial chromatin configuration, meiotic competence, and male pronucleus formation after intracytoplasmic sperm injection (ICSI) of equine oocytes.

机译：卵母细胞收集技术对马卵母细胞胞浆内精子注射（ICSI）后初始染色质构型，减数分裂能力和雄性核形成的影响。

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There is a great variability in the success of horse oocyte maturation and fertilization among laboratories. This study was conducted to determine if the meiotic and developmental competence of horse oocytes could be dependent on the method of oocyte collection, i.e., aspiration of follicular fluid with a vacuum apparatus, or opening follicles and scraping the granulosa layer. Horse oocytes were recovered from abattoir ovaries by aspiration or scraping and classified as having compact (Cp), expanded (Ex), or partial (P) cumuli. In Experiment 1 (Part A in May and Part B in October), oocytes were fixed immediately after collection to assess whether the collection method influenced the initial chromatin configuration of oocytes. In Experiment 2, in vitro maturation rates of oocytes recovered by aspiration or scraping were compared. In Experiment 3, oocytes were matured in vitro and submitted to intracytoplasmic sperm injection (ICSI). Initial chromatin configuration differed according to collection method in that there was a significantly higher prevalence of diffuse chromatin within the germinal vesicle in oocytes recovered by scraping than in oocytes recovered by aspiration (29/87, 33% and 28/166, 17%, respectively; P < 0.01). Maturation of oocytes to metaphase II did not significantly differ between scraped and aspirated oocytes (56/101, 55.4 % vs. 65/106, 61.4%, respectively). The overall pronucleus formation rate after ICSI of oocytes recovered by scraping was not significantly different than that of oocytes recovered by aspiration (50/99, 52.6% vs. 50/85, 68.5 %, respectively); however, the rate of abnormal fertilization was significantly higher for oocytes collected by aspiration (14/73, 19% vs. 6/94, 6%, respectively; P <0.05). These results demonstrate that the collection method affects the population of recovered oocytes and may contribute to differences in results observed among laboratories working with horse oocytes. Mol. Reprod. Dev. 60: 79-88, 2001. Copyright 2001 Wiley-Liss, Inc.
机译：在实验室之间，马卵母细胞成熟和受精的成功率存在很大差异。进行这项研究以确定马卵母细胞的减数分裂和发育能力是否取决于卵母细胞收集的方法，即用真空装置抽吸卵泡液，或打开卵泡并刮擦颗粒层。通过抽吸或刮擦从屠宰场卵巢中回收马卵母细胞，并归类为具有致密（Cp），扩张（Ex）或部分（P）卵丘。在实验1（5月的A部分和10月的B部分）中，卵母细胞在收集后立即固定，以评估收集方法是否影响了卵母细胞的初始染色质构型。在实验2中，比较了通过抽吸或刮擦回收的卵母细胞的体外成熟率。在实验3中，卵母细胞在体外成熟并接受胞浆内精子注射（ICSI）。初始染色质构型根据收集方法的不同而不同，因为通过刮擦回收的卵母细胞中生发囊泡中弥散染色质的发生率比通过抽吸回收的卵母细胞中的扩散染色质显着更高（分别为29 / 87、33％和28 / 166、17％） ; P <0.01）。刮除和吸出卵母细胞的卵母细胞成熟至中期II没有显着差异（分别为56 / 101、55.4％和65 / 106、61.4％）。通过刮除回收的卵母细胞的ICSI后总体原核形成率与通过抽吸回收的卵母细胞的总原核形成率没有显着差异（分别为50 / 99、52.6％和50 / 85、68.5％）;然而，通过抽吸收集的卵母细胞的异常受精率显着更高（分别为14 / 73、19％和6 / 94、6％; P <0.05）。这些结果表明，收集方法会影响回收的卵母细胞的数量，并且可能会导致在处理马卵母细胞的实验室之间观察到的结果差异。大声笑责备。开发人员60：79-88，2001。版权所有2001 Wiley-Liss，Inc.。

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