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首页> 外文期刊>Molecular reproduction and development >Novel sperm-binding proteins of epididymal origin contain four fibronectin type II-modules.
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Novel sperm-binding proteins of epididymal origin contain four fibronectin type II-modules.

机译：附睾来源的新型精子结合蛋白包含四个纤连蛋白II型模块。

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摘要

Novel fibronectin type II (Fn2)-module proteins were cloned from human and canine epididymal cDNA libraries. cDNA sequences predicted a highly conserved protein family, related but not homologous to ungulate seminal plasma proteins (approximately 50% sequence identity), and the first known examples of proteins with four tandemly arranged Fn2-domains. By Northern blot and in situ hybridization analyses the encoding mRNAs were shown to be abundant products of the epididymal duct epithelium, but not detectable in other tissues. Homologous mRNAs were identified in the epididymides of various mammals, representing members of this novel protein family of epididymal origin. Within the Fn2-module-encoding stretches, species homologues displayed >85% sequence identity, but showed high variability at their predicted N-termini. An antipeptide antiserum in Western blot analyses detected 30-35 kDa immunoreactive protein bands in epididymal tissue, cauda epididymidal fluid, and sperm membrane protein preparations. The tandem arrangement of increasing numbers of Fn2-modules might functionally correspond to the tendency to form oligomers that has been described for lipid-binding proteins.
机译：从人和犬附睾cDNA文库中克隆新型II型纤连蛋白（Fn2）模块蛋白。 cDNA序列预测了一个高度保守的蛋白家族，与有蹄类的精浆蛋白相关（但不同源）（约50％序列同一性），并且是具有四个串联排列的Fn2结构域的蛋白的第一个已知实例。通过Northern印迹和原位杂交分析，编码的mRNA显示为附睾导管上皮的丰富产物，但在其他组织中不可检测。在各种哺乳动物的附睾中鉴定出同源mRNA，代表该附睾起源的新蛋白家族的成员。在Fn2模块编码段内，物种同源物显示> 85％的序列同一性，但在其预测的N末端显示高变异性。 Western印迹分析中的抗肽抗血清检测到附睾组织，附睾液和精子膜蛋白制剂中的30-35 kDa免疫反应蛋白条带。 Fn2-模块数目增加的串联排列可能在功能上对应于已形成脂质结合蛋白的寡聚物的趋势。

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