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首页> 外文期刊>Mycologia >Genetic engineering of an ectomycorrhizal fungus Laccaria bicolor for use as a biological control agent.
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Genetic engineering of an ectomycorrhizal fungus Laccaria bicolor for use as a biological control agent.

机译：用作生物防治剂的外生菌根真菌双色紫胶菌的基因工程。

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Genetically engineered ectomycorrhizal fungi are being developed for use as biological control agents. Using the gold particle bombardment technique, Laccaria bicolor, an ectomycorrhizal fungus capable of forming ectomycorrhizae on a wide range of plant hosts was transformed. Structural gene sequences of the hygromycin B phosphotransferase (hph) gene (hygromycin resistance for selection of transformants), beta-glucuronidase (GUS) gene (reporter gene for tracking transformants) and Bacillus thuringiensis delta-endotoxin (BtCryIIIA) gene (Coleopteran specific insecticidal gene from Bacillus thuringiensis var. tenebrionis) were introduced into L. bicolor simultaneously. Southern and western blot analyses showed that the introduced sequences were inserted into the fungal genome and transcribed and translated. One of the transformants produced the insecticidal toxin; however, the levels were not sufficient to perform toxicity assays. The technique developed can be employed to generate superior strainsof L. bicolor suitable for use as biological control agents in various soil and plant conditions.
机译：基因工程的外生菌根真菌正在被开发用作生物防治剂。使用金粒子轰击技术，对能够在多种植物宿主上形成菌根的双歧杆菌（Laccaria bicolor）进行了转化。潮霉素B磷酸转移酶（hph）基因（潮霉素抗性用于选择转化子），β-葡萄糖醛酸糖苷酶（GUS）基因（用于追踪转化子的报告基因）和苏云金芽孢杆菌δ-内毒素（BtCryIIIA）基因（鞘翅目特异性杀虫基因）将来自苏云金芽孢杆菌（Bacillus thuringiensis var.tenebrionis）的植物同时引入双色乳杆菌。 Southern和Western印迹分析表明，引入的序列被插入到真菌基因组中并被转录和翻译。一种转化体产生了杀虫毒素。然而，该水平不足以进行毒性测定。所开发的技术可用于产生双色乳杆菌的优良菌株，适合在各种土壤和植物条件下用作生物防治剂。

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