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首页> 外文期刊>Mycologia >Increased conidiation associated with progression along the sterigmatocystin biosynthetic pathway.
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Increased conidiation associated with progression along the sterigmatocystin biosynthetic pathway.

机译：伴随着沿着胚藻毒素生物合成途径的进展而增加的分娩。

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摘要

The Aspergillus nidulans sterigmatocystin (ST) gene cluster contains both regulatory (aflR) and biosynthetic genes (stc genes) required for ST production. A total of 26 genes are in the cluster, 13 of which have been assigned a known function in the biosynthetic pathway. This complex secondary pathway represents a physiological cost to the fungus. We tested the amount of asexual spore production using a series of isogenic lines of A. nidulans, differing only in a mutation in aflR (resulting in a strain containing no ST intermediates) or a mutation in three stc genes that produced either no ST intermediates ( Delta stcJ), an early ST intermediate, norsoloroinic acid ( Delta stcE) or a late ST intermediate, versicolorin A ( Delta stcU). In two independently replicated experiments, we compared the numbers of conidia produced by each of these mutant strains and a wild type ST producer in a neutral (growth media) and a host (maize seed) environment. A stepwise increase in asexual spore production was observed with each progressive step in the ST pathway. Thus, the data suggest that recruitment or loss of these secondary metabolite pathway genes has a selective advantage apart from the physiological activity of the metabolite.
机译：构巢曲霉菌毒素（ST）基因簇包含ST生产所需的调节基因（aflR）和生物合成基因（stc基因）。簇中共有26个基因，其中13个在生物合成途径中被赋予了已知功能。这种复杂的次级途径代表了真菌的生理损失。我们使用构巢曲霉的一系列同基因品系测试了无性孢子产生的数量，这些区别仅在于aflR突变（导致菌株不含ST中间体）或三个stc基因突变（不产生ST中间体）导致突变（ Delta stcJ），早期的ST中间体，正七氢膦酸（Delta stcE）或晚期ST的中间体，Versicolorin A（Delta stcU）。在两个独立复制的实验中，我们比较了这些突变菌株和野生型ST生产者在中性（生长培养基）和宿主（玉米种子）环境中产生的分生孢子的数量。在ST通路中，每个进行性步骤均观察到无性孢子产量逐步增加。因此，数据表明这些次生代谢产物途径基因的募集或损失除代谢产物的生理活性外具有选择优势。

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