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首页> 外文期刊>Molecular reproduction and development >Estrogen-dependent expression of the tissue kallikrein gene (Klk1) in the mouse uterus and its implications for endometrial tissue growth.
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Estrogen-dependent expression of the tissue kallikrein gene (Klk1) in the mouse uterus and its implications for endometrial tissue growth.

机译：组织激肽释放酶基因（Klk1）在小鼠子宫中的雌激素依赖性表达及其对子宫内膜组织生长的影响。

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摘要

Tissue kallikrein mK1 is a serine protease involved in the generation of bioactive kinins for normal cardiac and arterial function in the mouse. In the present study, the tissue kallikrein gene Klk1, which codes for mK1, was shown to be one of the most prevalent of the Klk gene species in the uteri of adult mice, and its mRNA level was significantly higher at estrus than at diestrus. Klk1 mRNA expression was enhanced in the uteri of ovariectomized mice receiving estradiol-17beta treatment. Both endometrial epithelial and stromal cells isolated from the mice exhibited Klk1 expression at detectable levels when cultured in the presence of estradiol-17beta. mK1 was characterized using the recombinant active enzyme. mK1 had trypsin-like activity with a strong preference for Arg over Lys in the P1 position, and its activity was inhibited by typical serine protease inhibitors. Casein, gelatin, fibronectin, collagen type IV, and high-molecular-weight kininogen were degraded by mK1. The single-chain tissue-typeplasminogen activator was converted to the two-chain form by mK1. In addition, mK1 degraded insulin-like growth factor binding protein-3. The present data suggest that mK1 may be implicated in the growth of uterine endometrial tissues during the proliferative phase.
机译：组织激肽释放酶mK1是一种丝氨酸蛋白酶，参与小鼠正常心脏和动脉功能的生物活性激肽的产生。在本研究中，组织激肽释放酶基因Klk1（编码mK1）被证明是成年小鼠子宫中最流行的Klk基因之一，其发情期的mRNA水平显着高于发情期。在接受雌二醇-17β治疗的去卵巢小鼠的子宫中，Klk1 mRNA表达增强。从小鼠分离的子宫内膜上皮细胞和基质细胞在雌二醇-17β存在下培养时均以可检测的水平显示Klk1表达。使用重组活性酶表征mK1。 mK1具有胰蛋白酶样活性，比P1位置的Lys更偏向Arg，并且其活性被典型的丝氨酸蛋白酶抑制剂抑制。酪蛋白，明胶，纤连蛋白，IV型胶原和高分子量激肽原被mK1降解。单链组织型纤溶酶原激活剂通过mK1转化为两链形式。此外，mK1降解胰岛素样生长因子结合蛋白3。本数据表明，mK1可能与增生期子宫内膜组织的生长有关。

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