...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular reproduction and development >CDK5 is Present in Sea Urchin and Starfish Eggs and Embryos and Can Interact With p35, Cyclin E and Cyclin B3
【24h】

CDK5 is Present in Sea Urchin and Starfish Eggs and Embryos and Can Interact With p35, Cyclin E and Cyclin B3

机译：CDK5存在于海胆，海星卵和胚胎中，可与p35，Cyclin E和Cyclin B3相互作用

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kinase activity To investigate the potential for nonneuronal roles, we screened a starfish cDNA library with the yeast two-hybrid system, for possible CDK5 partners Interactions with clones expressing part of cyclin B3 and cyclin E proteins were found and the full-length cyclins were cloned These interactions were verified in vitro but not in extracts of starfish oocytes and embryos, at any stages, despite the presence of detectable amounts of CDK5, cyclin B3, and cyclin E We then looked for p35, the CDK5-specific activator, and cloned the sea urchin ortholog A sea urchin-specific anomaly in the amino acid sequence is the absence of N-terminal myristoylation signal, but nucleotide environment analysis suggests a much higher probability of translation initiation on the second methionine(Met44), that is associated with a conserved myristoylation signal p35 was found to associate with CDK5 and, when bacterially produced, to confer protein kinase activity to CDK5 immunoprecipitated from sea urchin eggs and embryos However, p35 mRNA expression was found to begin only at the end of the blastula stage, and the protein was undetectable at any embryonic stage, suggesting a neuronal role beginning in late larval stages
机译：尽管大多数细胞周期蛋白依赖性激酶（CDK）参与细胞周期控制，但CDK5在神经发生中的关键功能最为人所知。但是，我们从两细胞阶段cDNA文库中克隆了海胆CDK5，发现该蛋白存在于卵和胚胎，直至生殖期，但没有相关的激酶活性为了研究非神经元作用的潜力，我们用酵母双杂交系统筛选了海星cDNA文库，寻找可能的CDK5伴侣与表达部分细胞周期蛋白B3和细胞周期蛋白E的克隆的相互作用尽管存在可检测量的CDK5，cyclin B3和cyclin E，但仍在蛋白质中发现了蛋白并克隆了全长细胞周期蛋白，并在体外验证了这些相互作用，但并未在海星卵母细胞和胚胎的提取物中进行验证。 p35，CDK5特异性激活剂，并克隆了海胆直向同源物氨基酸序列中的海胆特异性异常是缺少N末端肉豆蔻酰化信号，但核潮汐环境分析表明，与保守的肉豆蔻酰化信号p35相关的第二个甲硫氨酸（Met44）的翻译起始可能性更高，被发现与CDK5相关，并且当细菌产生时，赋予蛋白激酶活性以从海上免疫沉淀的CDK5海胆卵和胚胎然而，发现p35 mRNA的表达仅在囊胚期结束时开始，并且在任何胚胎期均未检测到该蛋白，这表明神经元在幼虫后期开始发挥作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号