Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi

Tsuchiya D; Koga H; Taga M

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Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi

机译：丝状真菌有丝分裂核和染色体的扫描电镜观察

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A new method for scanning electron microscopy (SEM) of fungal mitotic nuclei and chromosomes was established for two ascomycetes, Cochliobolus heterostrophus and Neurospora crassa. Nuclei and chromosomes discharged from germling cells by tire germ-tube burst method were spread oil a surface of a glass slide. The spreads were impregnated with osmium-thiocarbohydrazide for conductive staining, followed by Coating With platinum, and observed by field-emission SEM. Ultrastructure of fungal chromosomes and nuclei was visualized by SEM for the first time.

机译：建立了两种子囊菌Cochliobolus heterostrophus和Neurospora crassa的真菌有丝分裂核和染色体扫描电子显微镜（SEM）的新方法。通过轮胎胚管破裂法从胚芽细胞中排出的细胞核和染色体在玻片表面上铺展。用s-硫代碳酰肼浸渍涂抹物以进行导电染色，然后用铂涂覆，并通过场发射SEM观察。 SEM首次观察到真菌染色体和核的超微结构。

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《Mycologia》 |2004年第2期|共3页
作者
Tsuchiya D; Koga H; Taga M;
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原文格式 PDF
正文语种 eng
中图分类 Q939.5;
关键词
Cochliobolus heterostrophus; Germ-tube burst method; Mitosis.;

机译：异翅球菌;菌管破裂法;有丝分裂;

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专利

1. Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi [J] . Tsuchiya D, Koga H, Taga M Mycologia . 2004,第2期

机译：丝状真菌有丝分裂核和染色体的扫描电镜观察
2. STEREO AND SCANNING ELECTRON MICROSCOPY CHARACTERISTICS OF POULTRY BREEDING BEETLE (Alphitobius diaperinus) a?? A FILAMENTOUS TOXIGENIC FUNGI CARRIER [J] . Carlos Eduardo da Silva Soares, André Weber, Vildes Maria Scussel Emirates Journal of Food and Agriculture . 2018,第2期

机译：家禽的甲虫（Alphitobius diaperinus）的立体和扫描电子显微镜特性丝状有毒真菌载体
3. Complementary visualization of mitotic barley chromatin by field-emission scanning electron microscopy and scanning force microscopy [J] . Schaper A., Formanek H., Jovin TM., Journal of Structural Biology . 2000,第1期

机译：场发射扫描电子显微镜和扫描力显微镜对有丝分裂大麦染色质的互补观察
4. The study of food- and air-borne fungi using environmental scanning electron microscopy [C] . M.L. Ng, E. K. Tang, L. N. Leong, Proceedings of International Kunming Symposium on Microscopy July 2-5, 2000 Kunming, Yunnan Province, China . 2000

机译：使用环境扫描电子显微镜研究食物和空气传播真菌
5. Simultaneous Bright-Field and Dark-Field Scanning Transmission Electron Microscopy in Scanning Electron Microscopy: A New Approach for Analyzing Polymer System Morphology. [D] . Patel, Binay S. 2013

机译：扫描电子显微镜中同时进行的明场和暗场扫描透射电子显微镜：一种分析聚合物系统形态的新方法。
6. Assembly of chromatin fibers into metaphase chromosomes analyzed by transmission electron microscopy and scanning electron microscopy. [O] . K W Adolph, L R Kreisman, R L Kuehn 1986

机译：通过透射电子显微镜和扫描电子显微镜分析染色质纤维组装成中期染色体。
7. High resolution analysis of mitotic metaphase chromosomes with scanning electron microscopy [O] . Schroeder-Reiter Elizabeth 2004

机译：用扫描电子显微镜分析有丝分裂中期染色体的高分辨率
8. Scanning Electron Microscopy Observations of the Effects of Hyperbaric Stress on the Populations of Segmented Filamentous Intestinal Flora on Normal Mice [R] . Merrell, B. R., Walker, R. I., Gillmore, J. D., 1979

机译：扫描电子显微镜观察高压应激对正常小鼠分段丝状肠道菌群的影响

Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi

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