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Cryptic species revealed by molecular phylogenetic analysis of sequences obtained from basidiomata of Tulasnella

机译：分子系统发育分析揭示了隐孢子虫的种类

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Delimitation of species and the search for a proper threshold for defining phylogenetic species in fungi are under discussion. In this study, morphological and molecular data are correlated to delimit species of Tulasnella, the most important mycobionts of Orchidaceae, which suffer from poor taxonomy. Resupinate basidiomata of Tulasnella species were collected in Ecuador and Germany, and 11 specimens (seven from Ecuador, four from Germany) were assigned to traditional species concepts by use of morphological keys. The specimens were compared by micro-anatomical examination with 75 specimens of Tulasnella borrowed from fungaria to obtain better insights on variation of characters. Sequences of the ITS region (127) were obtained after cloning from the fresh basidiomata and from pure cultures. Proportional variability of ITS sequences was analyzed within and among the cultures and the specimens designated to different morphospecies. Results suggested an intragenomic variation of less than 2%, an intraspecific variation of up to 4% and an interspecific divergence of more than 9% in Tulasnella. Cryptic species in Tulasnella, mostly from Ecuador, were revealed by phylogenetic analyses with 4% intraspecific divergence as a minimum threshold for delimiting species. Conventional diagnostic morphological characters appeared insufficient for species characterization. Arguments are presented for molecular delimitation of the established species Tulasnella albida, T. asymmetrica, T. eichleriana, T. cf. pinicola, T. tomaculum and T. violea
机译：物种定界和寻找合适的阈值以定义真菌中的系统发育物种正在讨论中。在这项研究中，形态学和分子数据与定殖杜兰属的物种有关，后者是兰科最重要的真菌，其分类学较差。在厄瓜多尔和德国收集了杜兰藻属种的恢复性basidiomata，通过形态键将11个标本（厄瓜多尔七个，德国四个）分配给传统物种概念。通过显微解剖检查将这些标本与从真菌中借来的75株杜氏藻的标本进行比较，以获得对字符变化的更好见解。从新鲜的basidiomata和纯培养物克隆后获得ITS区域的序列（127）。分析了ITS序列的比例变异性，并分析了不同形态种的培养物和标本之间的差异。结果表明，在图拉菌属中，基因组内变异小于2％，种内变异高达4％，种间差异大于9％。通过系统发育分析揭示了图拉斯奈拉的隐性物种，其中大部分来自厄瓜多尔，种内差异为4％，作为界定物种的最小阈值。传统的形态学诊断特征似乎不足以进行物种鉴定。提出的论点是对已建立的物种Tulasnella albida，T. asymmetrica，T. eichleriana，T. cf. pinicola，T。tomaculum和T. violea

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