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首页> 外文期刊>Mycologia >Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructans
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Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructans

机译：蝙蝠白鼻综合症：实时的TaqMan聚合酶链反应测试针对的是Geomyces destructans的基因间隔区

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摘要

The fungus Geomyces destructans is the causative agent of white-nose syndrome (WNS), a disease that has killed millions of North American hibernating bats. We describe a real-time TaqMan PCR test that detects DNA from G. destructans by targeting a portion of the multicopy intergenic spacer region of the rRNA gene complex. The test is highly sensitive, consistently detecting as little as 3.3 fg genomic DNA from G. destructans. The real-time PCR test specifically amplified genomic DNA from G. destructans but did not amplify target sequence from 54 closely related fungal isolates (including 43 Geomyces spp. isolates) associated with bats. The test was qualified further by analyzing DNA extracted from 91 bat wing skin samples, and PCR results matched histopathology findings. These data indicate the real-time TaqMan PCR method described herein is a sensitive, specific and rapid test to detect DNA from G. destructans and provides a valuable tool for WNS diagnostics and research.
机译：毁灭性真菌真菌是白鼻综合症（WNS）的病原体，该病已杀死数百万北美冬眠蝙蝠。我们描述了一种实时TaqMan PCR测试，该测试通过靶向rRNA基因复合体的多拷贝基因间间隔区的一部分来检测来自G. destructans的DNA。该测试具有很高的敏感性，可始终检测到来自解构芽孢杆菌的3.3 fg基因组DNA。实时PCR测试特异性地扩增了来自变形杆菌的基因组DNA，但没有从与蝙蝠相关的54个密切相关的真菌分离株（包括43个真菌霉属分离株）中扩增靶序列。通过分析从91个蝙蝠翼皮肤样本中提取的DNA，进一步验证了该测试的质量，PCR结果与组织病理学结果相符。这些数据表明，本文所述的实时TaqMan PCR方法是一种灵敏，特异和快速的检测方法，可检测来自变形链霉菌的DNA，并为WNS诊断和研究提供了有价值的工具。

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