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首页> 外文期刊>Molecular reproduction and development >Effects of aging on inositol 1,4,5-triphosphate-induced Ca2+ release in unfertilized mouse oocytes.
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Effects of aging on inositol 1,4,5-triphosphate-induced Ca2+ release in unfertilized mouse oocytes.

机译：衰老对未受精的小鼠卵母细胞中肌醇1,4,5-三磷酸诱导的Ca2 +释放的影响。

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We undertook the present study to examine the effects of oocyte aging on Ca2+ release from the inositol 1,4,5-triphosphate (InsP3)-sensitive Ca2+ channels of the endoplasmic reticulum membrane, because not only Ca2+ reuptake, but also Ca2+ release from the endoplasmic reticulum, substantially affect Ca2+ oscillations in fertilized oocytes. A transient increase in cytosolic free Ca2+ concentration ([Ca2+]i) was induced by photolysis of caged InsP3 microinjected into the cytoplasm of fresh (14 h postHCG) and aged (20 or 24 h post HCG) oocytes; the maximum rate of increase in [Ca2+]i significantly decreased in the aged oocytes. Reduced endoplasmic reticulum Ca2+ release in the aged oocyte may not be attributable to aging-related desensitization of the InsP3-sensitive Ca2+ channels in the endoplasmic reticulum because concentrations of caged InsP3 for half maximal [Ca2+]i increase were identical for fresh and aged oocytes. The peak [Ca2+]i response following administration of 5μM thapsigargin, a specific endoplasmic reticulum Ca2+-ATPase inhibitor, was significantly reduced in aged oocytes, suggesting reduction of the endoplasmic reticulum Ca2+ stores. It is concluded that reduction of Ca2+ release from the InsP3-sensitive Ca2+ stores in aged oocytes arises from depletion of the endoplasmic reticulum Ca2+ stores with aging. These aging-related changes in Ca2+ release and reuptake may account for alterations in Ca2+ oscillations in aged fertilized oocytes.
机译：我们进行了本研究，以检查卵母细胞衰老对内质网膜肌醇1,4,5-三磷酸（InsP3）敏感的Ca2 +通道中Ca2 +释放的影响，因为不仅Ca2 +吸收，而且Ca2 +从内质网释放。内质网实质上影响受精卵母细胞中的Ca2 +振荡。微注射入新鲜（HCG后14 h）和陈年（HCG后20或24 h）的细胞质的笼状InsP3的光解可诱导细胞内游离Ca 2+浓度（[Ca 2+] i）的瞬时增加;在老年卵母细胞中，[Ca2 +] i的最大增加速率显着降低。老化的卵母细胞中内质网Ca2 +释放的减少可能不归因于内质网中InsP3敏感的Ca2 +通道的衰老相关的脱敏，因为笼罩的InsP3浓度最大[Ca2 +] i的一半与新鲜和老化的卵母细胞相同。在老化的卵母细胞中，施用5μMthapsigargin（一种特异的内质网Ca2 + -ATPase抑制剂）后，峰值[Ca2 +] i响应显着降低，表明内质网Ca2 +存储量减少。结论是衰老的卵母细胞中InsP3敏感的Ca2 +存储区中Ca2 +释放的减少是由于内质网Ca2 +存储区的老化而引起的。这些与衰老相关的Ca2 +释放和再摄取变化可能解释了受精卵母细胞中Ca2 +振荡的变化。

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