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首页> 外文期刊>Mycologia >Confocal imaging of living fungal hyphae challenged with the fungal antagonist viscosinamide.
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Confocal imaging of living fungal hyphae challenged with the fungal antagonist viscosinamide.

机译：用真菌拮抗剂黏粘素酰胺攻击的活菌丝的共聚焦成像。

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The combination of confocal laser scanning microscopy (CLSM) and staining with vital fluorescence dyes has promising potential for studying subcellular structures in living filamentous fungi and oomycetes. CLSM offers high resolution images and thepossibility of optical sectioning of the specimen. Vital dyes make it possible to do this while the cells are still alive and with minimum disturbance of cellular processes. In this study confocal microscopy and the vital dyes Nile red, SYTO 13, DIOC7(3), and carboxy SNARF-1 were used on several species. Subsequently the technique was used to detect effects of the fungal antagonist viscosinamide. These fluorescent dyes were expected to stain hydrophobic elements, nuclei, mitochondria, and cytoplasm respectively. Nile red and DIOC7(3) were found to be especially well suited for confocal imaging. Nile red stained the vacuolar membranes in Rhizoctonia solani and demonstrated the presence of transvacuolar tubules. In addition, the stain showed that viscosinamide caused deformation of the hyphae. DIOC7(3) staining indicated that mitochondria lost their structural orientation upon exposure to viscosinamide. In conclusion, viscosinamide has effects on hyphae which can be detected with vital fluorescent dyes.The effects may be related to induction of cation channels.
机译：共聚焦激光扫描显微镜（CLSM）和重要的荧光染料染色的结合对于研究活的丝状真菌和卵菌的亚细胞结构具有广阔的前景。 CLSM可提供高分辨率图像，并可以进行标本的光学切片。重要的染料使得在细胞仍然活着并且对细胞过程的干扰最小的情况下可以做到这一点。在这项研究中，共聚焦显微镜和重要染料尼罗红，SYTO 13，DIOC7（3）和羧基SNARF-1被用于几种物种。随后，该技术被用于检测真菌拮抗剂粘二十酰胺的作用。这些荧光染料有望分别染色疏水元件，细胞核，线粒体和细胞质。尼罗红和DIOC7（3）特别适合共焦成像。尼罗河红染色了茄形假单胞菌中的液泡膜，并证实了存在的透空小管。另外，该污渍表明粘粘酰胺引起菌丝变形。 DIOC7（3）染色表明，线粒体在暴露于粘黏酰胺后丧失了结构取向。综上所述，粘黏酰胺对菌丝具有重要作用，而重要的荧光染料可以检测到这种作用，其作用可能与诱导阳离子通道有关。

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