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Genetic diversity of Phytophthora capsici isolates from pepper and pumpkin in Argentina

机译:阿根廷辣椒和南瓜中辣椒疫霉菌的遗传多样性

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摘要

Phytophthora capsici is a soilborne oomycete plant pathogen that limits pepper production worldwide. The population structure varies significantly depending on the location (e.g. Peru vs. USA) and little is known about the diversity of P. capsici in Argentina. Our objective was to assess the diversity of P. capsici in Argentina at key pepper production areas. Forty isolates were recovered 2006-2009 from pepper and one isolate from pumpkin at 11 locations. Isolates were assessed for mating type, mefenoxam sensitivity and multilocus single nucleotide polymorphism (SNP) genotype profiles. Ten isolates with identical SNP profiles also were genotyped with amplified fragment length polymorphism (AFLP) markers. All 41 isolates had the Al mating type and were sensitive to mefenoxam. Genotypic analysis using eight polymorphic SNP markers indicated 87% of the isolates had the same multilocus genotype, which is fixed for heterozygosity at seven of the eight SNP sites. AFLP analyses confirmed these findings, and overall it appears that clonal reproduction drives the population structure of P. capsici in Argentina. The implications for breeding resistant peppers and overall disease management are discussed.
机译:辣椒疫霉(Phytophthora capsici)是一种土壤传播的卵菌植物病原体,在世界范围内限制了胡椒的生产。人口结构因地理位置而异(例如,秘鲁与美国),并且人们对阿根廷辣椒疫霉的多样性知之甚少。我们的目标是评估阿根廷主要辣椒生产区的辣椒辣椒的多样性。 2006-2009年在11个地点从辣椒中回收了40种分离物,从南瓜中回收了一种分离物。评估了分离物的交配类型,甲氧西am敏感性和多基因座单核苷酸多态性(SNP)基因型谱。还使用扩增的片段长度多态性(AFLP)标记对十个具有相同SNP谱图的分离株进行基因分型。所有41个分离株均为Al交配型,对甲芬沙ox敏感。使用八个多态性SNP标记的基因型分析表明,有87%的分离株具有相同的多基因座基因型,该基因型在八个SNP位点中的七个固定为杂合性。 AFLP分析证实了这些发现,总体看来,克隆繁殖驱动了阿根廷辣椒疫霉菌的种群结构。讨论了抗性辣椒育种和总体疾病管理的意义。

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