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首页> 外文期刊>Mycologia >IS PENICILLIUM MONOPHYLETIC - AN EVALUATION OF PHYLOGENY IN THE FAMILY TRICHOCOMACEAE FROM 18S, 5.8S AND ITS RIBOSOMAL DNA SEQUENCE DATA
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IS PENICILLIUM MONOPHYLETIC - AN EVALUATION OF PHYLOGENY IN THE FAMILY TRICHOCOMACEAE FROM 18S, 5.8S AND ITS RIBOSOMAL DNA SEQUENCE DATA

机译:是青霉菌单鞭毛—从18S,5.8S及其家族核糖体DNA序列数据评估毛滴虫科的植物学

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摘要

Using ribosomal DNA sequences from 17 fungi, we evaluated the monophylly of Penicillium and the phylogeny in the ascomycete family Trichocomaceae (= Eurotiaceae). We determined the 5.8S and internal transcribed spacer rDNA sequences for 13 species, and the 18S rDNA sequences for Eupenicillium javanicum and Neosartorya fischeri. After including additional published 18S, 5.8S and ITS rDNA sequences, our data set consisted of 2204 aligned sites for 17 taxa and yielded 16 equally parsimonious trees, each requiring 609 nucleotide substitutions. Indicating that Penicillium is not monophyletic, all the trees show Eupenicillium javanicum (with a Penicillium state) grouping with the Aspergillus-producing taxa Neosartorya fischeri and Eurotium rubrum rather than the Penicillium-producing Talaromyces flavus var. macrosporus or Talaromyces bacillisporus. The branch separating Eupenicillium javanicum from Talaromyces flavus and Talaromyces bacillisporus received 98% bootstrap support. Providing further statistical evidence that Penicillium is not monophyletic, constraint trees forcing the penicillia to appear monophyletic were significantly less likely (using the maximum likelihood program DNAML) than trees allowing the penicillia to cluster into two groups, Aspergillus may be monophyletic, nested among species with Paecilomyces and Penicillium states. Monascus purpureus, although usually placed in a separate family, grouped with Eupenicillium and the Aspergillus species in the Trichocomaceae. Species in the Onygenales are basal to the Trichocomaceae. Coding regions varied little in length, were readily alignable and provided most of the evolutionary ''signal'' present in the data set, while the spacer regions varied in length from 309 to 447 base pairs and encompassed much unalignable data. We found evidence of higher levels of mutational saturation in the internal transcribed spacer regions than in the coding regions when we compared the pair-wise percent substitution, with and without correction for repeated substitutions at the same site, for the two data subsets. [References: 39]
机译:使用来自17种真菌的核糖体DNA序列,我们评估了青霉单孢菌和子囊菌科(Trichocomaceae)(= Eurotiaceae)的系统发育。我们确定了13个物种的5.8S和内部转录间隔区rDNA序列,以及爪哇大戟鱼和费氏新藻的18S rDNA序列。在包括其他已发布的18S,5.8S和ITS rDNA序列后,我们的数据集由2204个17个分类单元的比对位点组成,并产生了16个等简树,每个树需要609个核苷酸取代。指示青霉不是单生的,所有树木都显示爪哇大戟(具有青霉菌状态)与产曲霉的类群新Neo鱼和rub红(而不是产青霉的黄精变种)组合在一起。大型孢子或Talaromyces bacillisporus。将爪哇大戟鱼与黄褐藻和芽孢杆菌分离的分支获得了98%的自举支持。提供进一步的统计证据表明青霉菌不是单系的,强迫青霉菌出现单系的限制性树(使用最大似然程序DNAML)的可能性大大低于允许青霉菌聚类为两组的树,曲霉菌可能是单系的,嵌套在具有拟青霉和青霉菌状态。紫红曲菌,尽管通常被放置在一个单独的家族中,但与毛霉菌科中的杜仲和曲霉菌一起分组。 Onygenales的物种以毛囊科为基础。编码区的长度变化很小,易于对齐,并提供了数据集中存在的大多数进化“信号”,而间隔区的长度在309个碱基对至447个碱基对之间变化,并且包含了许多不可对齐的数据。当我们比较两个数据子集的成对百分比替换时,我们发现在内部转录的间隔区中的突变饱和度高于编码区,而在同一位置重复和不进行校正的情况下,我们比较了成对百分比替换。 [参考:39]

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