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首页> 外文期刊>Mycologia >Molecular characterization of airborne fungal spores in boreal forests of contrasting human disturbance.
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Molecular characterization of airborne fungal spores in boreal forests of contrasting human disturbance.

机译：与人类干扰相反的北方森林中空气传播的真菌孢子的分子特征。

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摘要

In this study we present a new approach to characterize fungal diversity with DNA sequencing of mycelium grown from trapped airborne spores. Fungal spores were extracted systematically from air in three boreal forest sites (clear-cut, young and old-growth forests) using an air sampling device. Internal transcribed spacer (ITS) sequences from the nuclear ribosomal DNA (nrDNA) were generated, and the sequences most likely taxon affinities were established through DNA homology searches. Phylogenetic analyses were used to classify similar sequences into operational taxonomic units (OTUs). The analyses indicated that a total of 84 different OTUs had been sampled, 24 basidiomycetes and 60 ascomycetes. OTUs belonging to the ascomycete orders Helotiales and Pleosporales were most frequent (31 and 18 respectively). A total of 54, 29 and 33 OTUs were sampled, respectively, in the old-growth, young and clear-cut forest sites. Although heavy generalization should be avoided due to few replicates, the results could indicate that old-growth boreal forests have significantly higher airborne fungal species richness than recently managed forests. The study shows that the spore-trapping approach has a great potential for targeting and studying anonymous fungi..
机译：在这项研究中，我们提出了一种新的方法来表征真菌的多样性，该方法利用从捕获的空气传播的孢子生长的菌丝体的DNA测序来表征真菌的多样性。使用空气采样装置从三个北方森林地带（清晰，年轻和古老的森林）中的空气中系统地提取了真菌孢子。从核糖体DNA（nrDNA）生成内部转录间隔区（ITS）序列，并通过DNA同源性搜索建立最可能的分类单元亲和力的序列。系统发育分析用于将相似序列分类为操作分类单位（OTU）。分析表明，总共采样了84种不同的OTU，24种担子菌和60种子囊菌。属于子囊菌属Helotiales和Pleosporales的OTU最常见（分别为31和18）。在老林，幼林和未砍伐林地分别采样了54、29和33个OTU。尽管由于重复次数少，应避免进行繁琐的概括，但结果可能表明，较早生长的北方森林比最近管理的森林具有更高的空气传播真菌物种丰富度。研究表明，诱集孢子的方法具有针对和研究匿名真菌的巨大潜力。

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