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首页> 外文期刊>Mycologia >RNA editing is absent in a single mitochondrial gene of Didymium iridis
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RNA editing is absent in a single mitochondrial gene of Didymium iridis

机译：鸢尾线虫的单个线粒体基因中缺少RNA编辑

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An open reading frame (ORF) was found in the mitochondrial genome of the Pan2-16 strain of Didymium iridis that showed high similarity to the NADH dehydrogenase subunit 3 (nad3) gene in other organisms. So far all other typical mitochondrial genes identified in this organism require RNA editing to generate ORFs capable of directing protein synthesis. The D. iridis sequence was compared to the putative nad3 gene in the related myxomycete Physarum polycephalum, which would require editing. Based on this comparison, editing sites could be predicted for the P. polycelphalum gene that would result in the synthesis of a highly conserved ND3 protein between the two organisms. To determine the editing status of the nad3 gene in other D. iridis strains, PCR was used to amplify this region from eight other independent isolates of the A1 Central American interbreeding series. In each case a 378 base pair ORF was detected by PCR amplification and sequencing. Three patterns of sequence variation were observed; however all base substitutions were in the third codon position and silent with respect to the amino acids encoded. The distribution of the sequence variants was mapped geographically. The requirement for RNA editing in all other typical mitochondrial genes of D. iridis and P. polycephalum and the presence of RNA editing in the nad3 gene of P. polycephalum suggest that the D. iridis nad3 gene might have been edited at one time. We propose that the D. iridis nad3 gene may have lost the requirement for RNA editing by reverse transcription of an edited transcript that subsequently was inserted into the genome.
机译：一个开放阅读框（ORF）被发现在虹吸双歧杆菌的Pan2-16菌株的线粒体基因组中，与其他生物中的NADH脱氢酶亚基3（nad3）基因具有高度相似性。到目前为止，在该生物体中鉴定出的所有其他典型线粒体基因都需要进行RNA编辑，以生成能够指导蛋白质合成的ORF。将D. iridis序列与相关的粘菌菌多头Physarum polycephalum中的推定的nad3基因进行比较，这需要进行编辑。基于此比较，可以预测聚丝孢菌基因的编辑位点，这将导致在两个生物之间合成高度保守的ND3蛋白。为了确定在其他D. iridis菌株中nad3基因的编辑状态，使用PCR从A1中美洲杂交系列的其他八个独立分离株中扩增该区域。在每种情况下，通过PCR扩增和测序检测到378个碱基对的ORF。观察到三种序列变异模式;然而，所有碱基取代均位于第三个密码子位置，并且相对于编码的氨基酸而言是沉默的。序列变体的分布在地理上作图。对所有其他典型的D. iridis和P. polycephalum线粒体基因进行RNA编辑的要求，以及在P. polycephalum的nad3基因中存在RNA编辑提示，D。iridis nad3基因可能已经被同时编辑过。我们建议，D。iridis nad3基因可能已经失去了对RNA编辑的要求，因为后来被插入基因组的已编辑转录本被逆转录了。

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