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首页> 外文期刊>Mycologia >SCREENING MARINE FUNGI FOR PLASMIDS AND CHARACTERIZATION OF A LINEAR MITOCHONDRIAL PLASMID IN A LULWORTHIA SP
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SCREENING MARINE FUNGI FOR PLASMIDS AND CHARACTERIZATION OF A LINEAR MITOCHONDRIAL PLASMID IN A LULWORTHIA SP

机译：Lulworthia SP中的线粒体筛查海洋真菌和表征线粒体线虫

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Twenty-one marine filamentous fungi, including 18 isolates capable of growth on hexadecane, were screened for plasmids. Twenty of the isolates screened did not have plasmids, indicating that neither the ability to grow on hydrocarbons nor the lack of this ability can be correlated with the presence of plasmids. One organism, Lulworthia sp. 046, that grows on hydrocarbons, contained a plasmid approximately 14kb in size. The plasmid, designated pQB, is linear, as shown by the results of both UV-nicking experiments and digestion with exonuclease III and lambda exonuclease. The plasmid was present in DNA extracted from mitochondria, and it banded with mitochondrial DNA when nuclear and mitochondrial DNA were separated on a cesium chloride gradient. These results suggest that the plasmid is mitochondrial in origin. Southern analysis showed that the plasmid is not homologous with nuclear or mitochondrial genomic DNA, and therefore replicates autonomously. [References: 47]
机译：筛选了二十一种海洋丝状真菌，包括18种能够在十六烷上生长的分离株，寻找质粒。筛选出的二十个分离株没有质粒，表明在碳氢化合物上生长的能力或缺乏这种能力都不能与质粒的存在相关。一种生物，Lulworthia sp。在碳氢化合物上生长的046质粒大小约为14kb。质粒被命名为pQB，是线性的，如紫外线切割实验以及用核酸外切酶III和λ核酸外切酶消化的结果所示。该质粒存在于从线粒体提取的DNA中，当在氯化铯梯度上分离核和线粒体DNA时，该质粒与线粒体DNA结合。这些结果表明该质粒起源于线粒体。 Southern分析表明该质粒与核或线粒体基因组DNA不同源，因此可以自主复制。 [参考：47]

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