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首页> 外文期刊>Mycologia >Ten polymorphic microsatellite loci identified from a small insert genomic library for Peronospora tabacina
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Ten polymorphic microsatellite loci identified from a small insert genomic library for Peronospora tabacina

机译：从一个小插入基因组文库中鉴定出十个多态微卫星位点

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摘要

Ten polymorphic microsatellite loci for the obligate biotrophic, oomycete pathogen of tobacco, Peronospora tabacina, were identified from a small insert genomic library enriched for GT motifs. Eighty-five percent of the 162 loci identified were composed of dinucleotide repeats, whereas only 4% and 11% were tri- and tetra-nucleotide repeats respectively. About 82% of all the microsatellites were perfect and within the library; only about 7% of the loci were duplicated. Primers were designed for 63 loci; 10 loci were polymorphic, 19 were monomorphic and 34 either failed to amplify or produced ambiguous/inconsistent results. The 10 polymorphic loci were characterized with 44 isolates of P. tabacina collected from tobacco plants growing in Europe, the Near East and North and South America. The number of alleles per locus was either three or four with a mean of 3.2, and the mean number of genotypes per locus was 3.6. Observed heterozygosity was 0.32-0.95, whereas expected heterozygosity was 0.44-0.69 for these loci. All loci except PT054 did not conform to the Hardy-Weinberg distribution. Polymorphic information content (PIC) for the loci was 0.35-0.69 with a mean of 0.50. These microsatellite loci provide a set of markers sufficient to perform genetic diversity and population studies of P. tabacina, and possibly other species of Peronospora.
机译：从一个富含GT序列的小型插入基因组文库中鉴定出十个多态性微卫星位点，用于烟草的专性生物营养性，卵菌病原体Peronospora tabacina。在确定的162个基因座中，有85％由二核苷酸重复组成，而分别只有4％和11％是三核苷酸和四核苷酸重复。所有微卫星中约有82％完美且位于图书馆内。仅重复了7％的基因座。引物设计用于63个基因座。 10个位点是多态的，19个位点是单态的，有34个扩增失败或产生了模棱两可/不一致的结果。这10个多态位点的特征是从欧洲，近东，北美洲和南美洲生长的烟草植物中收集到的44株烟草的分离株。每个基因座的等位基因数量为三个或四个，平均为3.2，每个基因座的基因型平均数量为3.6。对于这些基因座，观察到的杂合度为0.32-0.95，而预期杂合度为0.44-0.69。除PT054外，所有基因座均不符合Hardy-Weinberg分布。位点的多态信息含量（PIC）为0.35-0.69，平均值为0.50。这些微卫星基因座提供了足以进行烟粉假单胞菌以及可能的其他Peronospora物种的遗传多样性和种群研究的一组标记。

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