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首页> 外文期刊>Molecular reproduction and development >Variation of docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation: implications for sperm lipoperoxidative damage.
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Variation of docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation: implications for sperm lipoperoxidative damage.

机译：在不同成熟阶段的人类精子亚群中二十二碳六烯酸含量的变化：对精子脂过氧化损伤的影响。

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摘要

The oxidation of phospholipid-bound docosahexaenoic acid (DHA) has been shown to be one of the major factors that limit the motile life span of sperm in vitro. Sperm samples show high cell-to-cell variability in life span and, consequently, in susceptibility toward lipid peroxidation. Therefore, we postulated that there is also cell-to-cell variability in DHA concentration in human spermatozoa. In this study, the concentration of DHA in subsets of human spermatozoa isolated by a discontinuous Percoll density gradient was determined by gas chromatography. Four subsets of human spermatozoa were isolated using a discontinuous Percoll gradient: fraction 1 was enriched in immature germ cells and immature sperm, fractions 2 and 3 contained, mostly, immature sperm with cytoplasmic droplets, and fraction 4 contained, for the most part, morphologically normal sperm, as determined by histochemical analysis. The results indicated that there were significant differences in DHA content in sperm from all 4 fractions. DHA content in sperm from fraction 1 was 2.5-fold higher than that found in fraction 4. DHA content in mouse sperm obtained from the seminiferous tubules was 3-fold higher than that found in mouse sperm obtained from the epididymis, consistent with the findings observed in ejaculated human sperm. The results of this study indicate (i) there is cell-to-cell variability in the concentration of DHA in human sperm and (ii) that there is a net decrease in DHA content in sperm during the process of sperm maturation. Copyright 2000 Wiley-Liss, Inc.
机译：磷脂结合的二十二碳六烯酸（DHA）的氧化已被证明是限制精子在体外活动寿命的主要因素之一。精子样品在寿命中显示出很高的细胞间变异性，因此对脂质过氧化的敏感性较高。因此，我们推测人类精子中DHA浓度也存在细胞间差异。在这项研究中，通过气相色谱法测定了通过不连续的Percoll密度梯度分离的人类精子中DHA的浓度。使用不连续的Percoll梯度分离出四个子集的人类精子：馏分1富含未成熟的生殖细胞和未成熟的精子，馏分2和3大部分包含形态不成熟的带有细胞质液滴的精子，馏分4大部分在形态上通过组织化学分析确定的正常精子。结果表明，所有4个部分的精子中DHA含量均存在显着差异。馏分1中精子中的DHA含量比馏分4中高出2.5倍。从生精小管中获得的小鼠精子中DHA含量比附睾中获得的小鼠精子中的DHA高3倍，与观察到的结果一致在射精的人类精子中。这项研究的结果表明（i）人类精子中DHA的浓度存在细胞间差异，并且（ii）在精子成熟过程中，精子中DHA含量有净减少。版权所有2000 Wiley-Liss，Inc.

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