...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular reproduction and development >Expression of Penaeus monodon ortholog of Niemann-Pick type C-2 in the spermatic tract, and its role in sperm cholesterol removal
【24h】

Expression of Penaeus monodon ortholog of Niemann-Pick type C-2 in the spermatic tract, and its role in sperm cholesterol removal

机译：Niemann-Pick C-2型斑节对虾直系同源基因在精子中的表达及其在精子胆固醇清除中的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protein and lipid composition of sperm plasma membrane are modified as these gametes continue to mature during their transit along the spermatic tract. Our previous study revealed that during its journey through the spermatic duct of the black tiger prawn, Penaeus monodon, sperm cholesterol content decreases through the action of lipid-binding proteins within the luminal environment. In this study, the full cDNA sequence of epididymal secretory protein E1 (HE1), or Niemann-Pick C2 (NPC2), was cloned from P. monodon (termed Pmnpc2), and its conserved cholesterol/lipid-binding domain was characterized. The putative tertiary structure of PmNPC2 showed high similarity with the structure of Bos taurus NPC2. Pmnpc2 is expressed in many tissues, including the spermatic tract (i.e., testis, vas deferens, terminal ampoule) and the female thelycum. In situ hybridization revealed the presence of Pmnpc2 transcripts in the vas deferens, terminal ampoule, and thelycum epithelia, suggesting that PmNPC2 could be secreted into the lumen of the spermatic duct. A recombinant hexahistidine-tagged PmNPC2 (rPmNPC2-6His) was able to bind cholesterol and sperm lipid extracts, while co-incubation of sperm from the vas deferens with rPmNPC2-6His resulted in the depletion of cholesterol from these gametes. Together, these results suggest that PmNPC2 participates in sperm cholesterol efflux during the sperm maturation process in P. monodon. Mol. Reprod. Dev. 83: 259-270, 2016. (c) 2016 Wiley Periodicals, Inc.
机译：精子质膜的蛋白质和脂质组成被修饰，因为这些配子在沿着精管的运输过程中继续成熟。我们先前的研究表明，在黑虎虾对虾对虾的精子管中，精子胆固醇的含量通过在腔内环境中脂质结合蛋白的作用而降低。在这项研究中，附睾分泌蛋白E1（HE1）或Niemann-Pick C2（NPC2）的完整cDNA序列从斑节对虾中克隆（称为Pmnpc2），并对其保守的胆固醇/脂质结合结构域进行了表征。推测的PmNPC2三级结构与金牛座NPC2的结构高度相似。 Pmnpc2在许多组织中表达，包括精道（即睾丸，输精管，末端安瓿）和女性淋巴结。原位杂交揭示了输精管，末端安瓿和鞘膜上皮细胞中存在Pmnpc2转录本，表明PmNPC2可以分泌到精管的管腔中。重组六组氨酸标记的PmNPC2（rPmNPC2-6His）能够结合胆固醇和精子脂质提取物，而输精管的精子与rPmNPC2-6His共同孵育导致这些配子中的胆固醇消耗。总之，这些结果表明，PmNPC2在斑节对虾的精子成熟过程中参与了精子胆固醇外流。大声笑责备。开发人员83：259-270，2016。（c）2016 Wiley Periodicals，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号