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首页> 外文期刊>Molecular reproduction and development >Differential methylation status of IGF2-H19 locus does not affect the fertility of crossbred bulls but some of the CTCF binding sites could be potentially important.
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Differential methylation status of IGF2-H19 locus does not affect the fertility of crossbred bulls but some of the CTCF binding sites could be potentially important.

机译：IGF2-H19基因座的甲基化状态差异不会影响杂交公牛的繁殖力，但某些CTCF结合位点可能具有潜在的重要性。

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摘要

Associations between abnormal methylation of spermatozoan DNA with male infertility have been sought in recent years to identify a molecular explanation of differential spermatozoan function. The present work was undertaken to investigate the methylation profile of differentially methylated regions (DMRs) in the IGF2-H19 locus of Bos taurus x Bos indicus crossbred bull spermatozoa. Bulls having more than at least 100 insemination records over a period of 12 years were classified into two groups of five bulls each belonging to low- and high-fertility groups. The IGF2 and H19 DMR sequences in B. indicus cattle were observed to be in absolute homology with B. taurus cattle. The DNA of crossbred bull spermatozoa was isolated, bisulfite treated, and amplified for specific DMR regions using methylation-change-specific primers. The overall degree of methylation at IGF2-H19 DMRs was not found to be significantly different among two groups of bulls. The sixth CTCF binding site (CCCTC) identified in H19 DMR, however, had a significant methylation difference between the high- and low-fertility bulls. It was concluded that alteration of the methylation levels at IGF2-H19 DMRs might not be responsible for the fertility difference of crossbred bulls, although the role played by the specific CTCF binding sites at this locus, which could influence IGF2 expression during spermatogenesis and early embryonic development, deserves further attention.
机译：近年来一直在寻找精子DNA异常甲基化与男性不育之间的联系，以鉴定差异性精子功能的分子解释。目前的工作是为了研究金牛座x印度牛杂交牛精子的IGF2-H19基因座中差异甲基化区域（DMR）的甲基化分布。在过去的12年中，至少有100多个受精记录的公牛被分为两组，每组5头，分别属于低生育率和高生育率组。观察到印度双歧杆菌牛的IGF2和H19 DMR序列与金牛双歧杆菌牛绝对同源。分离杂交的公牛精子的DNA，用亚硫酸氢盐处理，并使用甲基化变化特异性引物扩增特定DMR区域。在两组公牛之间，IGF2-H19 DMR的总体甲基化程度没有显着差异。然而，H19 DMR中鉴定出的第六个CTCF结合位点（CCCTC）在高和低育性公牛之间有明显的甲基化差异。结论是，IGF2-H19 DMRs甲基化水平的改变可能不与杂交公牛的生育力差异有关，尽管该位点的特定CTCF结合位点发挥了作用，这可能影响精子发生和早期胚胎形成过程中IGF2的表达。发展，值得进一步关注。

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