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Cytopathological techniques for the diagnosis of glandular lesions of the genital tract.

机译:用于诊断生殖道腺体病变的细胞病理学技术。

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In spite of increasing standardization, some variations in preparation, fixation and staining methods still occur between different laboratories. A clear understanding of these techniques is mandatory to optimize the quality of cytological smears.Many ready-to-use commercial kits are now available that produce reliable and consistent staining; indeed, automation in various forms has improved efficiency in the cytopathol-ogy laboratory. New liquid-based technologies can produce monolayered cell samples, eliminate background and provide optimal concentration of epithelial cells. Some of these are based on vacuum filtration, others on density gradient centrifugation. In addition to conventional staining techniques, they also allow human papillomavirus (HPV) DNA testing, histochemistry, immunocytochemistry, and other special studies. The basic techniques of cytopreparation are summarized in this chapter with an overview of laboratory processing.
机译:尽管标准化程度不断提高,但不同实验室之间的制备,固定和染色方法仍存在一些差异。必须对这些技术有清楚的了解,以优化细胞学涂片的质量。现在有许多现成的可立即使用的商业试剂盒,可产生可靠且一致的染色。实际上,在细胞病理学实验室中,各种形式的自动化已提高了效率。新的基于液体的技术可以产生单层细胞样品,消除背景并提供上皮细胞的最佳浓度。其中一些基于真空过滤,而另一些基于密度梯度离心。除了常规的染色技术外,它们还可以进行人乳头瘤病毒(HPV)DNA测试,组织化学,免疫细胞化学和其他特殊研究。本章概述了细胞制备的基本技术,并对实验室处理进行了概述。

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