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首页> 外文期刊>Cancer: A Journal of the American Cancer Society >Performance of p16INK4a immunocytochemistry as a marker of anal squamous intraepithelial lesions.
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Performance of p16INK4a immunocytochemistry as a marker of anal squamous intraepithelial lesions.

机译：p16INK4a免疫细胞化学作为肛门鳞状上皮内病变标记物的性能。

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BACKGROUND: Protein p16(INK4a) immunocytochemistry (ICCp16) has the potential to reveal lesions at risk of progression to anal cancer. This study examined measures of diagnostic validity of ICCp16 in HIV-positive patients treated at the Tropical Medicine Foundation of Amazonas in the coloproctology outpatient clinic. METHODS: One hundred ninety HIV-positive patients were consecutively enrolled in 2007 and 2008. All patients underwent anal cytologic sampling to perform ICCp16 in conventional and GluCyte (Synermed International, Westfield, Indiana and S ao Paulo, Brazil) smears and also for genotyping of human papillomavirus (HPV). Patients were then subjected to anal biopsies monitored by high-resolution anoscopy. Hematoxylin-eosin and immunoperoxidase p16 (clone 6H12) stains were performed in slides with biopsied and cytological specimens, respectively. HPV genotyping on anal scrapings was performed by a polymerase-chain reaction (PCR)-based method. The immunochemical findings were compared with histopathological and PCR results in contingency tables and analyzed by nonparametric tests. Measures of diagnostic validity of ICCp16 were calculated. Statistical significance was set at P .05). In the best scenario, ICCp16 presented 31% sensitivity and 81% specificity for the diagnosis of anal squamous intraepithelial lesion (ASIL) and 30% and 66%, respectively, for the diagnosis of infection with high-risk HPV. CONCLUSIONS: There was no association between ICCp16 results and histopathological findings nor between ICCp16 and HPV genotyping. ICCp16 showed poor sensitivity and moderate specificity for the diagnosis of ASIL or high-risk HPV.
机译：背景：蛋白p16（INK4a）免疫细胞化学（ICCp16）有潜力揭示有发展为肛门癌风险的病变的潜力。这项研究检查了ICCp16在亚热带热带亚马孙州热带医学基金会在阴道内科门诊治疗的HIV阳性患者中的诊断有效性。方法：在2007年和2008年连续入选了190例HIV阳性患者。所有患者均接受了肛门细胞学采样，以常规和GluCyte（Synermed International，Westfield，印第安纳州和巴西圣保罗）涂片进行ICCp16检测，并对他们的基因分型人乳头瘤病毒（HPV）。然后对患者进行高分辨率活检监测的肛门活检。在载有活检标本和细胞学标本的载玻片上分别进行苏木精-曙红和免疫过氧化物酶p16（克隆6H12）染色。通过基于聚合酶链反应（PCR）的方法对肛门刮屑进行HPV基因分型。将免疫化学结果与列联表中的组织病理学和PCR结果进行比较，并通过非参数测试进行分析。计算了ICCp16的诊断有效性的量度。统计显着性设为P 0.05）。在最佳情况下，ICCp16诊断肛门鳞状上皮内病变（ASIL）的敏感性为31％，特异性为81％，诊断高危HPV感染的敏感性分别为30％和66％。结论：ICCp16结果与组织病理学结果之间，ICCp16与HPV基因分型之间均无关联。 ICCp16对ASIL或高危HPV的诊断显示出较差的敏感性和中等特异性。

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