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首页> 外文期刊>Knee surgery, sports traumatology, arthroscopy: official journal of the ESSKA >Effects of radiofrequency energy on porcine articular cartilage: Higher-power settings in ablation mode show lower thermal radiation injury
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Effects of radiofrequency energy on porcine articular cartilage: Higher-power settings in ablation mode show lower thermal radiation injury

机译：射频能量对猪关节软骨的影响：消融模式下的较高功率设置显示较低的热辐射损伤

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Purpose: The purpose of this study was to compare the radiofrequency (RF) injury effect on cartilage in the different settings that are mostly used in clinical work under rigidly controlled laboratory conditions. Methods: Twelve fresh porcine knees were used in our study. Five treatment areas were created on the femoral condyles of each knee: the control group, coagulation (setting 2) group, coagulation (setting 7) group, ablation (setting 2) group, and ablation (setting 7) group. Hematoxylin/eosin staining, dual fluorescence staining, and the GAG content were observed to evaluate the histological cartilage changes, vacuolar cell rate of chondrocytes, depth of chondrocyte death, and detection of GAG content. Results: Vacuolar cell rates of chondrocytes in each experimental group were higher than that in the control group (P < 0. 05); there was no significant difference in vacuolar cell rate among experimental groups. Dual fluorescent staining showed that the ablation (setting 7) group had a smaller depth of cell death than did the coagulation (setting 2) group (P < 0. 05); the other experimental groups showed no statistically significant difference (n. s.). In addition, there was no significant difference in GAG content between the experimental groups and control group (n. s.). Conclusions: The coagulation mode results in heavier thermal radiation injury to chondrocytes than does the ablation mode. Higher-power settings in the ablation mode result in lower thermal radiation injury and may be most suitable for cartilage debridement.
机译：目的：本研究的目的是比较在严格控制的实验室条件下临床工作中最常使用的不同环境中射频（RF）对软骨的损伤作用。方法：本研究使用十二个新鲜的猪膝盖。在每个膝盖的股骨dy上创建五个治疗区域：对照组，凝结（设置2）组，凝结（设置7）组，消融（设置2）组和消融（设置7）组。观察苏木精/曙红染色，双重荧光染色和GAG含量，以评估组织学软骨变化，软骨细胞的液泡细胞率，软骨细胞死亡深度以及GAG含量的检测。结果：各实验组软骨细胞的空泡细胞率均高于对照组（P <0。05）。实验组间空泡细胞率无明显差异。双重荧光染色显示，消融（设置7）组的细胞死亡深度比凝血（设置2）组小（P <0. 05）。其他实验组无统计学差异（n。s。）。此外，实验组和对照组之间的GAG含量没有显着差异（n。s。）。结论：凝结方式比消融方式对软骨细胞的热辐射损伤更大。消融模式下的较高功率设置可减少热辐射伤害，并且可能最适合于软骨清创术。

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