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首页> 外文期刊>Korean Journal of Applied Entomology >Molecular Identification of Trichogramma(Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis
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Molecular Identification of Trichogramma(Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

机译：基于ITS2 rDNA序列分析的亚洲玉米Ost毛线虫膜鳞茎虫卵寄生虫的分子鉴定。

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摘要

To identify the species of Trichogramma occurring in the com fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3'terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were dividedinto three groups (K-1, -2, and -3). Evolutionary distances {d) within and between groups based on ITS2 sequences were estimated to be ^ 0.005 and > 0.080, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. conjusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dmdrolimi, respectively. However, K-3 was clustered with three different species, namely, T. conjitsum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. conjusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. conjusum, and T. chilonis. On the basis of these results, we infer that the speciesof collected in this study are closely related to T. ostriniae (K-l) and T. dendrolimi(K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.
机译：为了鉴定在朝鲜玉米田中出现的赤眼蜂属的物种，将其作为福氏玉米Ost的卵寄生虫，我们对本研究中收集到的112个寄生虫的ITS2核rDNA全长进行了测序。作为区分物种的参考，我们还从NCBI GenBank数据库中检索了60个赤眼蜂属物种的全长ITS2序列。根据ITS2序列的大小和3'末端序列模式，将本研究中收集的赤眼蜂样品分为三组（K-1，-2和-3）。基于ITS2序列的组内和组间进化距离{d）分别估计为^ 0.005和> 0.080。在各组或种之间的净平均距离中，K-1和T. ostriniae，K-2和T. dendrolimi以及K-3和T. conjusum之间的d值最低，分别为0.016、0.001和1.0。分别为0.002。在系统发育树中，K-1和K-2分别与ostriniae和Dmdrolimi聚在一起。但是，K-3聚集有三种不同的物种，即尖吻锥虫，chilonis锥虫和bilingensis锥虫。 NCBI BLAST结果显示，属于K-1和K-2的寄生虫分别与ostriniae和T. dendrolimi具有99％的同一性。从洪川市收集的K-3中的寄生虫与弓形虫和拟南芥有99-100％的同源性，从高昌市收集的K-3寄生虫中的一种与寄生虫，弓形虫和拟南芥有98％的同源性。 。根据这些结果，我们推断本研究中收集到的菌种与曲霉（K-1）和树突线虫（K-2）密切相关。但是，不可能仅使用ITS2序列来区分K-3物种。

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