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首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Optimization of Culture Conditions of Bacillus pumilus JB-1 for Chungkook-jang Fermentation in Soybean Boiling-Waste Liquor Medium
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Optimization of Culture Conditions of Bacillus pumilus JB-1 for Chungkook-jang Fermentation in Soybean Boiling-Waste Liquor Medium

机译：短程芽孢杆菌JB-1在大豆沸腾废液培养基中发酵Chukkook-jang发酵的培养条件的优化

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Department of Food Science and Technology Kyungpook National University, Daegu 702-701, Korea -Soybean is useful source of protein, especially in Asia. But soybean needs heat inactivation or fermentation process before consumption, since it contains the toxic lectin and various protease inhibitors. Therefore, production of soybean boiling-waste liquor (SB WL) as a byproduct is inevitable. In this study, the chemical composition of SBWL and the optimization of culture conditions for Bacillus pumilus JB-1, a selected strain for functional chungkuk-jang fermentation, using SBWL were investigated. The SBWL contains 88% water, 9.5% free sugar, 1.6% crude protein, 0.3% crude fat, 0.1% crude fiber and 2.1% ash, respectively. The contents of total polyphenol, total flavonoids and free amino acid in SBWL were 55%, 76%, and 30% of those of raw soybean, respectively. Culture conditions for B. pumilus JB-1 in SBWL were optimized. The 1/10-diluted, 0.1% of (NH-4)2SO_4added SBWL without pH adjustment and carbonsource addition was cultured at 37deg C for 48 h with agitation (120 rpm). The 0.5% of inoculation was enough. The large scale fermentation in 5-L jar fermentor showed that the SBWL is a good resource for production of chungkuk-jang starter and functional ingredients.
机译：庆北国立大学食品科学与技术系，韩国大邱702-701-大豆是有用的蛋白质来源，尤其是在亚洲。但是大豆需要食用前进行热灭活或发酵，因为它含有有毒的凝集素和各种蛋白酶抑制剂。因此，不可避免地产生副产物大豆沸腾废液（SB WL）。在这项研究中，研究了SBWL的化学组成和短小芽孢杆菌JB-1的培养条件的优化，BBillus pumilus JB-1是使用SBWL进行功能性忠姜发酵的一种选择菌株。 SBWL分别包含88％的水，9.5％的游离糖，1.6％的粗蛋白，0.3％的粗脂肪，0.1％的粗纤维和2.1％的灰分。 SBWL中总多酚，总黄酮和游离氨基酸的含量分别为生大豆的55％，76％和30％。优化了SBWL中短小芽孢杆菌JB-1的培养条件。在不调节pH和添加碳源的情况下，将1/10稀释的0.1％的（NH-4）2SO_4添加的SBWL于37°C搅拌（120 rpm）培养48小时。接种0.5％就足够了。 5-L罐发酵罐的大规模发酵表明，SBWL是生产忠国酱发酵剂和功能性成分的良好资源。

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