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首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Isolation and Characterization of Plant-Derived Lactic Acid Bacteria as Potential Probiotic
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Isolation and Characterization of Plant-Derived Lactic Acid Bacteria as Potential Probiotic

机译：植物乳酸菌作为潜在益生菌的分离与鉴定

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Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and 02 were isolated from Kimchi and onion, and identified as Lactobacillus plantation on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum 02 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum 02 also showedhigh bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomo-nas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2-98.2%) in MRS broth containing 10% skim milk. Antioxidantactivity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8-61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum 02 may be potential probiotic startercultures with applications with fermentative products.
机译：从植物相关的发酵食品和农作物中分离出植物乳酸菌，并对其益生菌性质进行了研究。从泡菜和洋葱中分离出K27和O 2菌株，并根据16S rRNA基因分析将其鉴定为乳酸杆菌种植园。这两个菌株对酸（pH 2.5的MRS肉汤）具有高度抗性，其中植物乳杆菌K27和植物乳杆菌02的存活率分别为90.2％和97.3％。植物乳杆菌K27和植物乳杆菌02还显示出对0.5％的奥加高的高胆汁抗性，存活率超过70％。他们显示出对大肠杆菌KCCM 40880和铜绿假单胞菌ATCC 10145致病菌株的抑制作用。这两种菌株的抗菌作用可能是由于乳酸的存在。在MRS肉汤中，两种菌株的ACE抑制活性范围为72.8％至80.6％。值得注意的是，这两种菌株在含有10％脱脂乳的MRS肉汤中显示出较高的ACE抑制活性（89.2-98.2％）。通过DPPH自由基清除活性来测试抗氧化活性，菌株的抗氧化活性在56.8-61.5％的范围内。在这项研究中获得的结果表明，植物乳杆菌K27和植物乳杆菌02可能是潜在的益生菌发酵剂，与发酵产品一起使用。

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